Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

Figure 3

Comparison of theoretical plate numbers and peak asymmetry obtained on XB-C18 column for 20% of acetonitrile and 30% of methanol: (A) acetonitrile and 10 mM water solution of ammonium acetate adjusted to pH 4 with acetic acid; (B) acetonitrile and 10 mM water solution of ammonium formate adjusted to pH 4 with formic acid; (C) methanol and 10 mM water solution of ammonium acetate adjusted to pH 3 with acetic acid; (D) methanol and 10 mM water solution of ammonium acetate adjusted to pH 4 with acetic acid.