Systematic Evaluation of Chromatographic Parameters for Isoquinoline Alkaloids on XB-C18 Core-Shell Column Using Different Mobile Phase Compositions

Table 2

The comparison of retention times and peak resolutions of investigated alkaloids in 35% of methanol in water at different pH and ammonium acetate concentrations.

	pH = 3				pH = 4
	20 mM		10 mM		20 mM		10 mM
	tR	RS	tR	RS	tR	RS	tR	RS
Protopine	8.05	0.63	8.34	0.77	10.67	1.12	10.51	1.10
Allocryptopine	8.47	0.96	8.92	0.76	11.56	2.25	11.23	2.36
Chelidonine	9.11	1.18	9.49	1.18	13.37	0.08	13.15	0.30
Coptisine	9.68	10.60	10.18	10.02	13.43	10.48	13.37	12.94
Berberine	15.88	2.57	17.23	2.75	23.82	7.93	23.9	14.47
Sanguinarine	17.73	13.12	19.47	13.26	36.85	6.21	48.31	5.71
Chelerythrine	29.85	—	33.4	—	50.16	—	63.02	—