SRM-MS Method Development for Hepcidin-25 Peptide

Table 1

Relative ionization value of hepcidin-M to hepcidin-I according to charge state.

Charge state	Hepcidin-I		Hepcidin-M		Relative ionization value (hepcidin-M/I)
	m/z	Area	m/z	Area
2	1394.52	9.7E + 07	1626.64	3.8E + 08	3.92
3	930.02	1.3E + 09	1084.76	3.0E + 09	2.34
4	697.76	9.0E + 09	813.82	8.4E + 09	0.93
5	558.41	8.7E + 09	651.26	2.3E + 10	2.69
6	465.51	8.9E + 07	542.88	3.7E + 09	41.76
Sum of area		1.9E + 10		3.9E + 10	2.03