Simultaneous Determination of Over-the-Counter Pain Relievers in Commercial Pharmaceutical Products Utilizing Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) Multivariate Calibration Model

Table 5

Determination of the studied drugs in commercial tablets by the MCR-ALS model by the proposed and the reported HPLC method.

		MCR-ALS	HPLC
Sample 1	PAR
	(Mean + SD)	99.22 ± 1.11	99.86 ± 0.86
	t	0.86	—
	F	1.69	—
	CAF
	(Mean + SD)	99.74 ± 0.72	99.8 ± 0.70
	t	0.50	—
	F	1.08	—
Sample 2	PAR
	(Mean + SD)	99.06 ± 0.96	99.6 ± 0.83
	t	1.61	—
	F	1.35	—
	CAF
	(Mean + SD)	99.14 ± 1.28	99.0 ± 1.44
	t	0.09
	F	0.79
Sample 3	PAR
	(Mean + SD)	100.1 ± 1.11	100.5 ± 1.27
	t	0.80	—
	F	0.76	—
Sample 4	PAR
	(Mean + SD)	99.5 ± 1.45	100.5 ± 1.47
	t	1.07	-
	F	0.97	-
Sample 5	NPX
	(Mean + SD)	98.7 ± 1.50	99.9 ± 1.25
	t	2.67	—
	F	1.44	—
Sample 6	IPF
	(Mean + SD)	99.6 ± 1.77	99.1 ± 1.49
	t	0.59	—
	F	1.40	—
Sample 7	ASA
	(Mean + SD)	100.2 ± 1.85	99.9 ± 1.58
	t	0.34	—
	F	1.37	—
Sample 8	PAR
	(Mean + SD)	100.82 ± 1.34	100.4 ± 1.45
	t	0.35	—
	F	0.85	—
Sample 9	NPX
	(Mean + SD)	99.7 ± 1.55	100.4 ± 1.45
	t	0.93	—
	F	1.14	—
Sample 10	IPF
	(Mean + SD)	100.2 ± 1.68	99.5 ± 1.54
	t	0.56	—
	F	1.19	—

The reference HPLC published method used C₁₈ (250 × 4.6 mm, 5.0 μm) column at 35°C, and the mobile phase composed of 15 mM phosphate buffer (pH 3.25) and acetonitrile using gradient elution at 1.1 mL·min⁻¹ flow rate. SD: standard deviation of the mean of the percentage recovery from the label claim amount for 5 determinations. Theoretical values for t and F at are 2.78 and 6.39, respectively.