Design and Synthesis of Ag Nanocluster Molecular Beacon for Adenosine Triphosphate Detection

Figure 3

(a) Synthesis time of AgNCs in 2, 4, 6, 8, 12, 24, and 48 hours. (b) Effect of T4 DNA ligase reaction time on fluorescence intensity. The reaction times are 0, 5, 10, 20, 30, 40, 60, and 90 min. (c) Effect of T4 DNA ligase dosage on fluorescence intensity. Ligase dosage of 0, 2.5, 5, 7.5, 10, 12.5, and 15 U. (d) Effect of pH values on fluorescence intensity. pH values of 6.6, 7.0, 7.4, 7.8, and 8.2.

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