Design and Synthesis of Ag Nanocluster Molecular Beacon for Adenosine Triphosphate Detection

Figure 4

(a) Fluorescence spectra of MB-Ag nanoclusters for ATP detection. The ATP concentrations are (from A to J) 0, 0.01, 0.1, 0.5, 1, 4, 7, 10, 30, and 50 μmol·L⁻¹. (b) Linear relationship between ATP concentration and fluorescence intensity. Conditions: 1 μmol·L⁻¹ DNA; 6 μmol·L⁻¹ Ag; 6 μmol·L⁻¹ NaBH₄; 10 U T4 DNA ligase; and 50 mmol·L⁻¹ Tris buffer (pH 7.4).

(a)

(b)