Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation

Table 5

Calibration curves, linearity ranges, limit of detection (LOD), limit of quantification (LOQ), precision, repeatability, and stability of the twelve analytes.

Analytes	Calibration curves	R^2	Linear range (μg/mL)	Precision (RSD, %)		LOQ (ng/mL)	LOD (ng/mL)	Repeatability (RSD, %, n = 6)	Stability (RSD, %, n = 6)
				Intraday	Interday
(1) THCA	Y = 6928.5X + 233.51	0.9998	0.22–8.90	1.78	3.13	2.22	0.56	4.05	2.64
(2) THDCA	Y = 7196.9X + 2433.4	0.9996	0.68–27.20	0.62	2.52	2.72	0.68	4.12	2.72
(3) TCA	Y = 2615.2X + 70.046	0.9997	0.27–10.60	2.33	3.40	5.30	1.33	3.29	4.25
(4) GHCA	Y = 3991.9X + 233.63	0.9989	0.14–2.80	2.86	3.11	5.60	1.40	3.57	4.19
(5) GCA	Y = 57819X + 4483.4	0.9982	0.15–3.00	0.82	1.42	0.75	0.19	4.04	2.35
(6) GHDCA	Y = 9643.4X + 652.74	0.9990	0.16–3.15	0.79	1.96	3.15	0.79	3.54	1.71
(7) TCDCA	Y = 2210.5X + 223.69	0.9997	0.55–21.90	1.93	3.34	6.84	2.19	3.43	2.99
(8) HCA	Y = 10575X + 110.15	0.9994	0.20–7.80	2.18	4.25	2.17	0.78	3.54	3.90
(9) CA	Y = 2408.1X + 4.2004	0.9991	0.16–3.25	2.99	4.01	8.13	1.63	3.27	3.97
(10) GCDCA	Y = 7134.9X + 1131.2	0.9987	0.49–9.85	1.13	1.53	4.93	1.23	4.11	2.19
(11) HDCA	Y = 25067X + 2257.9	0.9989	0.22–4.40	0.85	2.43	8.80	2.20	3.65	3.82
(12) CDCA	Y = 5537.9X + 920.54	0.9985	0.42–8.45	1.49	1.98	84.50	28.17	3.81	1.78