Quantitative Characteristics of Toxic Compounds According to the Solvent Type
Table 2
Preparation of working standards (WSs) containing three target compounds (benzene, toluene, and MIT) based on four different solvents (MeOH, DMSO, hexane, and PBS).
(a) Reagent grade chemical (RGC)
Compound name
Benzene
Toluene
MIT
Concentration (%)
99.5
99.5
95
Density (g·mL−1)
0.878
0.867
1.35
(b-1) The first primary standard (PS-1)
Compound
Benzene
Toluene
MeOH
Volume (μL)
20
20
1,960
Dilution fraction
0.010
0.010
Concentration (ng·μL−1)
8,736
8,627
(b-2) The second primary standard (PS-2)
Compound
MIT
Mass (mg)
180
Volume (mL)
2.000
Concentration (ng·μL−1)
90,000
(c) The first working standard (WS)
Working standard
PS-1
PS-2
Solventa
Compound
Benzene
Toluene
MIT
Volume (μL)
100
100
1,800
Dilution fraction
0.05
0.05
Concentration (ng·μL−1)
416
411
4,286
(d) The final working standard at 5 concentration levels
Order
Mixing volume (μL)
Dilution fraction
Concentration (ng·μL−1)
1st L-WS
Solvent
Benzene
Toluene
MIT
1
40
1,960
0.020
8.32
8.22
85.7
2
100
1,900
0.050
20.8
20.5
214
3
200
1,800
0.100
41.6
41.1
429
4
400
1,600
0.20
83.2
82.2
857
5
1,000
1,000
0.50
208
205
2,143
aFour solvents were used in this study: (1) MeOH, (2) DMSO, (3) hexane, and (4) PBS.
