A Sensitive Fluorescence Biosensor for Silver Ions (Ag⁺) Detection Based on C-Ag⁺-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification

Figure 2

Fluorescence emission spectra of different solutions: (a) 10 nmol/L oligo-1 + 1500 pmol/L Ag⁺; (b) 40 nmol/L oligo-2 + 1500 pmol/L Ag⁺; (c) 10 nmol/L oligo-1 + 40 nmol/L oligo-2; (d) 10 nmol/L oligo-1 + 40 nmol/L oligo-2 + 10 U Exo III; (e) 10 nmol/L oligo-1 + 40 nmol/L oligo-2 + 10 U Exo III + 1500 pmol/L Ag⁺. Experimental conditions: 50 mmol/L K⁺ and 160 nmol/L NMM.