Research Article

A Sensitive Fluorescence Biosensor for Silver Ions (Ag+) Detection Based on C-Ag+-C Structure and Exonuclease III-Assisted Dual-Recycling Amplification

Figure 3

Optimization of experimental conditions: (a) effect of the incubation time of Exo III digestion; (b) effect of Exo III concentration; (c) effect of signal DNA concentration; (d) effect of the incubation temperature; (e) effect of the signal DNA/capture DNA ratio; (f) effect of the pH value. Experimental conditions: 10 nmol/L oligo-1, 40 nmol/L oligo-2, 10 U Exo III, 50 mmol/L K+, and 160 nmol/L NMM. Error bars represent the standard deviation of three independent experiments.
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