Rapid-Response and Highly Sensitive Boronate Derivative-Based Fluorescence Probe for Detecting H₂O₂ in Living Cells

Figure 5

Fluorescence emission spectra of TPIOP boronate after reacting with (a) 15 μM H₂O₂ and 15 mM of other ROS and (b) 15 μM H₂O₂ along with 15 mM of other ROS. (c) Bar chart of selectivity and interferences of TPIOP boronate. Experimental conditions: 2 μM of TPIOP boronate in 10 mM HEPES buffer solution (pH 7.4 and 2 vol% DMSO). (1) TPIOP probe, (2) H₂O₂, (3) KO₂, (4) , (5) TBHP, (6) mCPBA, (7) HOCl, (8) , (9) , (10) , (11) ONOO⁻, and (12) BPO, respectively. Insets: corresponding fluorescence images.

(a)

(b)

(c)