Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum

Table 1

Biotin-FBP and FA-HRP concentration optimization.

Biotin-FPB (μg/mL)	0.52			0.26			0.13
FA-HRP (μg/ml)	1	0.5	0.25	1	0.5	0.25	1	0.5	0.25
Standard 1 (0 ng/mL)	1065638	652382	224155	633363	353217	129995	417738	217887	79858
Standard 2 (2.5 ng/mL)	980613	537333	208843	509083	288071	98061	319829	137721	52073
Standard 3 (5.0 ng/mL)	687334	439226	167008	377044	198141	70016	208496	113568	30422
Standard 4 (10.0 ng/mL)	556606	325643	102205	240137	117481	40667	111972	55471	16877
Standard 5 (25.0 ng/mL)	258853	121479	41827	84444	34271	8641	24247	9238	1727