Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats

Table 1

MS/MS transitions and parameters for detection of the analytes and internal standards.

Analytes	MRM	Cone voltage (V)	Collision energy (eV)
Prim-O-glucosylcimifugin	468.40 ⟶ 307.32	60	30
Cimifugin	307.34 ⟶ 259.23	55	20
4′-O-β-D-glucosyl-5-O-methylvisamminol	453.47 ⟶ 291.30	55	30
5-O-methylvisamminol	291.31 ⟶ 209.13	52	20
sec-O-glucosylhamaudol	439.38 ⟶ 277.28	28	16
Puerarin (IS)	417.29 ⟶ 73.05	32	28