Research Article

A Simple, Rapid, Fluorometric Assay for Dopamine by In Situ Reaction of Boronic Acids and cis-Diol

Figure 4

The effect of experimental conditions on the fluorescence intensity: (a) pH, (b) the ratio of 3-HPBA to DA, (c) reaction time, and (d) temperature. Other parameters were fixed, and 0.8 mM 3-HPBA and 100 μM DA reacted in 10 mM Na2CO3.
(a)
(b)
(c)
(d)