Research Article
Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component
Figure 6
Separation of the mixture of flavonoids and phenolic acids with the use of HPTLC (a) and PPEC (b) techniques. Experiment conditions: the mobile phase: butanol 1.2 M, acetonitrile 10% v/v, aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate), and 0.040 M of SDS; the stationary phase: HPTLC RP-18W. PPEC: polarization voltage 1400 V; experiment time 8 min; experiment temperature 38oC. The order of solutes for HPTLC: (1) rutin, (2) hesperidin; (3) trans-cinnamic acid and hesperidin; (4) caffeic acid and o-coumaric acid; (5) flavanone; (6) vanillic acid. The order of compounds in the PPEC experiment: (1) flavanone; (2) trans-cinnamic acid and hesperetin; (3) o-coumaric acid; (4) rutin; (5) hesperidin; (6) vanillic acid; (7) caffeic acid.
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