Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

Figure 7

Separation of plant extracts with HPTLC (a) and PPEC (b) techniques. Plant extracts No.: (1) Carduus carlinoides herb, (2) Carduus carlinoides flowers, (3) Carduus carlinoides inflorescentia methanolic extract fraction Fb, (4) Carduus nigrescens flowers, (5) Carduus nigrescens flowers methanolic extract fraction Fb, (6) Cirsium canum flowers, (7) Cirsum deccusatum flowers. Experiment conditions: the mobile phase: 0.040 M SDS, pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate), 1.2 M butanol, and 10% v/v acetonitrile; the stationary phase: HPTLC RP-18W. PPEC experiment: polarization voltage 1400 V; experiment time 8 min. Detection after derivatization at λ = 366 nm.

(a)

(b)