Research Article

Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method

Figure 2

Quantification of P1, P2, and P3 in PCME by HPTLC at λ = 460 nm (mobile phase: dichloromethane : methanol : formic acid (90 : 9.5 : 0.5)). (a) Chromatogram of standard P3 (Rf = 0.34), P2 (Rf = 0.47), and P1 (Rf = 0.65). (b) 3D display of all tracks. (c) Chromatogram of PCME (P3, spot 6, Rf = 0.34; P2, spot 7, Rf = 0.47; P1, spot 8, Rf = 0.65). (d) Spectral comparison of all the tracks at 460 nm.
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