Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method

Table 5

R_f and linear regression data for the calibration curve of P1, P2, and P3 (n = 6).

Parameters	P1	P2	P3
Linearity range (ng/spot)	100–1200	100–1200	100–1200
Regression equation	Y = 2.725X + 122.44	Y = 1.675X + 755.87	Y = 2.008X + 211.51
Correlation (r^2) coefficient	0.993 ± 0.001	0.993 ± 0.001	0.9972 ± 0.001
Slope ± SD	2.725 ± 0.025	1.675 ± 0.014	2.008 ± 0.007
Intercept ± SD	122.44 ± 9.30	755.87 ± 10.07	211.51 ± 12.75
Standard error of slope	0.005	0.006	0.003
Standard error of intercept	3.795	4.11	5.206
Rf	0.65 ± 0.004	0.47 ± 0.003	0.34 ± 0.001
LOD (ng)	31.04	29.23	12.33
LOQ (ng)	94.06	88.58	37.36