Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method

Table 6

Recovery as accuracy studies of the proposed HPTLC method (n = 6).

Percent of P1, P2, and P3 added to analyte	Theoretical concentration of P1, P2, and P3 (ng/band)	P1			P2			P3
		Concentration found (ng/band) ± SD	%RSD	% recovery	Concentration found (ng/band) ± SD	%RSD	% recovery	Concentration found (ng/band) ± SD	%RSD	% recovery
0	200	197.78 ± 2.57	1.29	98.89	198.14 ± 2.79	1.41	99.07	196.72 ± 2.38	1.21	98.36
50	300	296.07 ± 3.72	1.25	98.69	296.65 ± 4.37	1.47	98.88	297.66 ± 3.67	1.23	99.22
100	400	398.88 ± 5.31	1.33	99.72	396.25 ± 5.91	1.49	99.06	396.17 ± 5.29	1.33	99.04
150	500	495.02 ± 6.73	1.35	99.01	492.85 ± 7.54	1.52	98.57	494.28 ± 6.73	1.36	98.85