Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant Plicosepalus curviflorus Using HPTLC Validated Method

Table 8

Robustness of the proposed HPTLC method (n = 6).

Optimization condition	P1		P2		P3
	SD	%RSD	SD	%RSD	SD	%RSD
Mobile phase composition (dichloromethane : methanol : FA = 90 : 9.5 : 0.5)
90 : 9.5 : 0.5	3.51	1.18	3.31	1.12	4.26	1.42
89.5 : 10 : 0.5	3.57	1.19	3.37	1.15	4.29	1.44
90.5 : 9 : 0.5	3.62	1.21	3.41	1.18	4.37	1.46
Mobile phase volume (for saturation)
18 mL	3.34	1.13	3.62	1.23	4.17	1.40
20 mL	3.38	1.14	3.67	1.25	4.23	1.43
22 mL	3.42	1.16	3.69	1.27	4.31	1.45
Duration of saturation
10 min	3.45	1.16	3.21	1.08	4.01	1.35
20 min	3.49	1.18	3.26	1.09	4.03	1.36
30 min	3.52	1.19	3.31	1.10	4.09	1.38