Biological Safety Studies and Simultaneous Determination of Linagliptin and Synthetic Impurities by LC-PDA

Table 2

Percentage of degradation of LGT under different stress conditions to evaluate the selectivity of the analytical method.

Exposition time (h)	Conditions evaluated
	UVA radiation	30% (v/v) hydrogen peroxide	Basic thermal (1 mol·L^−1 at 80°C)	Acid thermal (1 mol·L^−1 at 80°C)	Thermal (80°C)	Acid (1 mol·L^−1)	Basic (1 mol·L^−1)
0.25	—	—	17.14	—	—	—	—
0.5	—	—	31.26	—	—	—	—
0.75	—	—	44.24	—	—	—	—
1	4.36	—	58.74	—	9.37	—	—
1.5	—	—	74.14	—	—	—	—
2	7.75	6.87	86.57	9.04	10.00	—	—
3	13.56	—	—	—	8.31	—	—
4	34.86	8.65	—	9.89	—	—	—
6	—	8.73	—	11.11	—	—	—
10	—	9.85	—	15.50	—	—	—
24	—	—	—	—	—	3.12	12.06
48	—	—	—	—	—	—	23.19