Research Article
Pharmacokinetic Study and Tissue Distribution of Lorlatinib in Mouse Serum and Tissue Samples by Liquid Chromatography-Mass Spectrometry
Table 3
Accuracy and precision for samples (within-day
; between-day
).
| Samples | Concentration spiked (ng/ml) | Within-day accuracy (%) | Within-day precision (%) | Between-day accuracy (%) | Between-day precision (%) |
| Serum | 5.00 | 3.87 | 2.99 | 10.17 | 10.46 | 10.00 | −1.92 | 5.04 | 0.68 | 7.60 | 100.00 | 2.33 | 5.01 | 0.52 | 7.01 | 800.00 | 2.01 | 4.06 | −0.39 | 7.18 |
| Lung | 5.00 | 4.83 | 4.57 | 0.41 | 4.86 | 10.00 | 8.35 | 7.23 | 6.34 | 4.82 | 100.00 | 3.62 | 4.45 | 3.70 | 3.38 | 400.00 | 1.96 | 4.61 | 2.56 | 3.36 |
| Heart | 5.00 | −3.53 | 6.15 | −5.31 | 5.13 | 10.00 | 7.78 | 5.51 | 6.77 | 4.27 | 100.00 | 6.31 | 4.79 | 8.20 | 5.54 | 400.00 | 5.12 | 4.70 | 7.68 | 4.19 |
| Liver | 5.00 | 1.07 | 4.07 | 0.07 | 7.91 | 10.00 | 4.23 | 4.77 | 8.84 | 7.11 | 100.00 | 6.93 | 2.50 | 8.84 | 4.36 | 400.00 | 4.91 | 6.14 | 5.06 | 4.26 |
| Stomach | 5.00 | −12.17 | 5.13 | −6.02 | 8.52 | 10.00 | 0.23 | 8.24 | 2.46 | 5.04 | 100.00 | 1.29 | 4.01 | −0.16 | 4.03 | 400.00 | −3.37 | 3.25 | −2.98 | 3.53 |
| Spleen | 5.00 | −5.27 | 6.96 | 1.22 | 6.71 | 10.00 | 6.47 | 2.86 | 7.94 | 4.52 | 100.00 | 3.66 | 7.83 | 2.74 | 6.42 | 400.00 | 5.12 | 3.11 | 1.21 | 5.18 |
| Brain | 5.00 | −1.33 | 4.04 | −0.61 | 5.12 | 10.00 | 6.50 | 4.33 | 0.68 | 10.38 | 100.00 | 6.07 | 7.66 | 0.35 | 10.98 | 400.00 | 5.50 | 1.69 | −0.42 | 8.09 |
| Kidney | 5.00 | −1.73 | 4.42 | −0.82 | 8.38 | 10.00 | 2.27 | 3.20 | 5.91 | 5.04 | 100.00 | −3.31 | 5.43 | 1.50 | 5.24 | 400.00 | −4.68 | 5.53 | 1.26 | 6.64 |
| Large intestine | 5.00 | −7.47 | 6.75 | −0.94 | 19.73 | 10.00 | −0.72 | 6.82 | −3.37 | 9.60 | 100.00 | 3.20 | 3.70 | −6.42 | 8.89 | 400.00 | 0.12 | 2.78 | −4.90 | 8.00 |
| Small intestine | 5.00 | 2.5 | 5.26 | −7.1 | 9.26 | 10.00 | 3.03 | 2.38 | −0.48 | 7.79 | 100.00 | −1.13 | 3.42 | −0.89 | 3.58 | 400.00 | −3.43 | 6.30 | −1.78 | 4.35 |
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