Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

Table 1

LOD and LOQ values of spironolactone examined in the form of combined pharmaceutical formulations using various analytical methods.

Method	Drug content	LOD and LOQ	Reference
UV-Vis spectrophotometry	Spironolactone + hydrochlorothiazide	LOD: 0.300 (μg/mL)	[3]
		LOQ: 0.800 (μg/mL)
HP-TLC with densitometry	Spironolactone + torsemide	LOD: 0.024 (μg/spot)	[4]
		LOQ: 0.070 (μg/spot)
HP-TLC with UV-Vis detection	Spironolactone + furosemide	LOD: 0.040 (ng/mL)	[5]
		LOQ: 0.160 (ng/mL)
TLC with densitometry	Spironolactone + hydrochlorothiazide	LOD: 0.090 (μg/spot)	[6]
		LOQ: 0.280 (μg/spot)
HP-TLC with densitometry	Spironolactone + metolazone	LOD: 0.200 (μg/spot)	[7]
		LOQ: 0.600 (μg/spot)
RP-HPLC	Spironolactone + furosemide	LOD: 1.240 (μg/mL)	[8]
		LOQ: 3.760 (μg/mL)
RP-HPLC	Spironolactone + frusemide	LOD: 0.0002 (μg/mL)	[9]
		LOQ: 0.0006 (μg/mL)
RP-HPLC	Spironolactone + hydrochlorothiazide	LOD: 0.100 (μg/mL)	[3]
		LOQ: 0.200 (μg/mL)
RP-HPLC	Spironolactone + hydrochlorothiazide	LOD: 0.850 (μg/mL)	[7]
		LOQ: 2.560 (μg/mL)