Journal of Analytical Methods in Chemistry

Research Article

Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

Table 2

Quantity of spironolactone applied on chromatographic plates for NP-TLC and RP-TLC developed by different mobile phases.


Chromatographic system	Sorbent	Mobile phase	Quantity of spironolactone applied on chromatographic plates (µg/spot)

NP-TLC	1.05715	A	0.2–0.4
		B	0.2–0.4
		C	0.6–0.8
	1.05567	A	0.3–0.5
		B	0.5–0.7
		C	1.0–3.0
	1.05554	A	0.6–0.8
		B	0.2–0.4
		C	1.0–3.0

RP-TLC	RP-2F₂₅₄	D	0.2–0.4
		E	0.2–0.4
		F	2.0–4.0
	RP-18F₂₅₄	D	1.0–3.0
		E	0.2–0.4
		F	1.0–3.0
	RP-8F₂₅₄	D	2.0–4.0
		E	2.0–4.0
		F	1.0–3.0

Mobile phase A: n-hexane-ethyl acetate-glacial acetic acid (24.5 : 24.5 : 1, v/v/v); mobile phase B: chloroform-acetone (45 : 5, v/v); mobile phase C: ethyl acetate-n-hexane (38 : 12, v/v); mobile phase D: methanol-water (40 : 10, v/v); mobile phase E: acetonitrile-water (35 : 15, v/v); mobile phase F: dioxane-water (40 : 10, v/v).