Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

Table 3

Limits of detection and quantitation of spironolactone obtained by means of the NP-TLC method in different chromatographic conditions.

NP-TLC method
Sorbent	Mobile phase	LOD^1	LOD^2	Average value of LOD	LOQ^1	LOQ^2	Average value of LOQ
		µg/spot			µg/spot
1.05715	A	0.052	0.115	0.084	0.159	0.350	0.254
	B	0.043	0.095	0.069	0.131	0.287	0.209
	C	0.063	0.313	0.188	0.190	0.948	0.569
1.05567	A	0.018	0.051	0.034	0.053	0.153	0.103
	B	0.044	0.189	0.116	0.134	0.573	0.354
	C	0.514	0.785	0.650	1.558	2.380	1.969
1.05554	A	0.055	0.274	0.164	0.166	0.829	0.498
	B	0.041	0.090	0.066	0.124	0.272	0.198
	C	0.505	0.772	0.638	1.531	2.338	1.934

Mobile phase A: n-hexane-ethyl acetate-glacial acetic acid (24.5 : 24.5 : 1, v/v/v); mobile phase B: chloroform-acetone (45 : 5, v/v); mobile phase C: ethyl acetate-n-hexane (38 : 12, v/v); ¹the value calculated on the basis of residual standard deviation of the appropriate calibration curve; ²the value calculated on the basis of standard deviation of the intercept of the appropriate calibration curve.