Journal of Analytical Methods in Chemistry

Research Article

Influence of Different Chromatographic Conditions on the Level of Detection and Quantitation of Spironolactone by means of TLC-Densitometry

Table 4

Limits of detection and quantification of spironolactone obtained by means of the RP-TLC method in different chromatographic conditions.


RP-TLC method
Sorbent	Mobile phase	LOD¹	LOD²	Average value of LOD	LOQ¹	LOQ²	Average value of LOQ
Sorbent	Mobile phase	µg/spot			µg/spot

RP-2F₂₅₄	D	0.050	0.111	0.080	0.154	0.338	0.246
	E	0.063	0.138	0.100	0.190	0.417	0.304
	F	0.590	1.297	0.944	1.788	3.932	2.860

RP-18F₂₅₄	D	0.365	0.558	0.462	1.107	1.691	1.399
	E	0.063	0.138	0.100	0.191	0.419	0.305
	F	0.590	0.901	0.746	1.787	2.730	2.258

RP-8F₂₅₄	D	0.627	1.379	1.003	1.900	4.178	3.039
	E	0.674	1.483	1.078	2.044	4.494	3.269
	F	0.560	0.856	0.708	1.698	2.593	2.146

Mobile phase D: methanol-water (40 : 10, v/v); mobile phase E: acetonitrile-water (35 : 15, v/v); mobile phase F: dioxane-water (40 : 10, v/v); ¹the value calculated on the basis of residual standard deviation of the appropriate calibration curve; ²the value calculated on the basis of standard deviation of the intercept of the appropriate calibration curve.