Fingerprint Spectrophotometric Methods for the Determination of Co-Formulated Otic Solution of Ciprofloxacin and Fluocinolone Acetonide in Their Challengeable Ratio

Table 4

Statistical comparison between the results obtained by the proposed spectrophotometric methods and official methods [6] for the determination of CIP and FLU in Otovel® otic solution.

Methods	CIP							FLU
	a-Centering		EXRS	CM	UCS	SS	BP^a	a-Centering		RS	BP^a
	NS	FS						NS	FS
Mean	100.03	99.81	99.66	99.44	99.61	99.89	99.85	100.27	99.45	99.37	99.73
SD	0.56	0.36	0.54	0.61	0.52	0.64	0.32	1.20	1.31	1.21	0.61
Variance	0.31	0.13	0.29	0.37	0.27	0.42	0.10	1.45	1.73	1.46	0.37
N	6	6	6	6	6	6	6	6	6	6	6
t-Test^b	0.86	0.18	0.73	1.46	0.96	0.14	—	0.97	0.48	0.66	—
F value^c	3.01	1.27	2.80	3.55	2.63	4	—	3.87	4.61	3.90	—

^aThe BP method for (CIP) is HPLC, while ^athe BP method for FLU is the absorption method. ^bThe corresponding tabulated value of Student’s t-test is equal to 2.23 at . ^cThe corresponding tabulated value of F is equal to 5.05 at . NS results were obtained by using the normalized spectrum. FS results were obtained by using the factorized spectrum.