Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

Table 7

Tiliroside contents in extracts from Tilia L (±SD, ).

Samples	Efficiency of extraction (%)^a	Content of tiliroside (mg per g dry extracts)
MAC-E70%	13.7	1.530 ± 0.001
MACst-H	17.4	0.142 ± 0.005
MACst-E50%	14.6	1.105 ± 0.010
MACst-E70%	17.4	1.717 ± 0.005
MACst-E100%	5.3	1.289 ± 0.002
MACst-E70% + AA0.5%	18.5	5.010 ± 0.024
MACst-E70% + AA1%	18.4	5.370 ± 0.033
MACst-E70% + AA2%	20.1	2.870 ± 0.011
MACst-E70% + AA5%	21.1	2.540 ± 0.010
MACst-E70% + AA10%	19.5	2.990 ± 0.009
MACst-E70% + DE5%	15.4	3.900 ± 0.013
MACst-E70% + DE10%	13.2	3.560 ± 0.012
MACst-E100% + AA0.5%	6	2.130 ± 0.008
MACst-E100% + AA1%	6	2.160 ± 0.006
ASE-M100% (80)	14.8	4.980 ± 0.037
ASE-M70% (80)	22.3	4.440 ± 0.028
ASE-M50% (80)	22.0	1.371 ± 0.013
ASE-CH100% (50)	1.5	0.193 ± 0.001
ASE-E100% (80)	3.3	6.810 ± 0.014
ASE-E70% (80)	19.6	2.980 ± 0.007
ASE-E50% (80)	8.8	2.320 ± 0.012
ASE-E70% (80) + AA0.1%	23.0	3.200 ± 0.013
ASE-E70% (80) + AA0.5%	18.9	5.160 ± 0.017
ASE-E70% (80) + AA1%	26.4	7.400 ± 0.019
ASE-E70% (80) + AA5%	27.8	1.802 ± 0.004
ASE-E100% (80) + AA0.5%	18.9	5.850 ± 0.012
ASE-E100% (80) + AA1%	10.8	5.560 ± 0.015
UR-E70%	23.6	1.225 ± 0.006
UR-DE	5.6	9.842 ± 0.028
UR-E70% + AA1%	18.1	1.821 ± 0.010
UAE-M100% (rt)	3.6	4.281 ± 0.084
UAE-M70% (rt)	5.1	1.358 ± 0.016
UAE-M50% (rt)	6.6	0.829 ± 0.004
UAE-E100% (rt)	2.5	3.291 ± 0.059
UAE-E70% (rt)	4.0	1.728 ± 0.028
UAE-E50% (rt)	4.1	1.244 ± 0.019
UAE-M100% (80)	9.4	2.116 ± 0.038
UAE-M70% (80)	9.2	1.120 ± 0.019
UAE-M50% (80)	5.4	1.650 ± 0.054
UAE-E100% (80)	7	2.406 ± 0.001
UAE-E70% (80)	7.5	1.607 ± 0.007
UAE-E50% (80)	5.8	2.072 ± 0.015
UAE-E70% (80) + AA0.5%	8.4	1.848 ± 0.004
UAE-E70% (80) + AA1%	6.6	3.212 ± 0.021
UAE-E100% (80) + AA0.5%	2.8	3.322 ± 0.012
UAE-E100% (80) + AA1%	4	3.368 ± 0.057

^aCalculated as percentage of dry extract obtained from 1 g of raw material.