Simultaneous Determination of Drugs Affecting Central Nervous System (CNS) in Bulk and Pharmaceutical Formulations Using Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS)

Table 1

The concentration matrix used for the preparation of the calibration and validation sets of CHZ, CRZ, HAL, IMP, and PTN.

Sample no.	Calibration set (μg/mL)					Validation set (μg/mL)
	HAL	IMP	CHZ	CRZ	PTN	HAL	IMP	CHZ	CRZ	PTN
1	5.3	5.3	2.7	7.7	11.5	9.0	7.0	0.5	13.0	17.0
2	5.3	0.5	0.3	15.0	7.3	7.0	5.0	4.5	1.0	5.0
3	0.5	0.5	5.0	4.0	20.0	5.0	9.0	4.5	4.0	13.5
4	0.5	10.0	1.5	15.0	11.5	9.0	9.0	0.5	10.0	5.0
5	10	2.9	5.0	7.7	7.3	9.0	1.0	3.5	4.0	10.0
6	2.9	10.0	2.7	4.0	7.3	1.0	7.0	0.5	7.0	13.5
7	10.0	5.3	1.5	4.0	15.8	7.0	1.0	2.5	10.0	13.5
8	5.3	2.9	1.5	11.4	20.0	1.0	5.0	3.5	1.0	6.5
9	2.9	2.9	3.9	15.0	15.8	5.0	7.0	3.5	4.0	5.0
10	2.9	7.7	5.0	11.4	11.5	7.0	7.0	1.5	1.0	6.5
11	7.7	10.0	3.9	7.7	20.0	7.0	3.0	0.5	4.0	10.0
12	10.0	7.7	2.7	15.0	20.0	3.0	1.0	1.5	7.0	5.0
13	7.7	5.3	5.0	15.0	3.0	1.0	3.0	2.5	13.0	5.0
14	5.3	10.0	5.0	0.3	15.8	3.0	5.0	0.5	4.0	17.0
15	10.0	10.0	0.3	11.4	3.0	5.0	1.0	2.5	13.0	6.5
16	10.0	0.5	3.9	0.3	11.5
17	0.5	7.7	0.3	7.7	15.8
18	7.7	0.5	2.7	11.4	15.8
19	0.5	5.3	3.9	11.4	7.3
20	5.3	7.7	3.9	4.0	3.0
21	7.7	7.7	1.5	0.3	7.3
22	7.7	2.9	0.3	4.0	11.5
23	2.9	0.5	1.5	7.7	3.0
24	0.5	2.9	2.7	0.3	3.0
25	2.9	5.3	0.3	0.3	20.0