Determination of Isosorbide-5-Mononitrate in Human Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Bioequivalence Study

Table 2

Intraday and interday accuracy and precision of quality control samples of 5-ISMN.

Nominal con. (ng/ml)	Intraday (n = 6)			Interday (n = 18)
	Measured con. (mean ± SD, ng/mL)	Precision (RSD %)	Accuracy (%)	Measured con. (mean ± SD, ng/mL)	Precision (RSD %)	Accuracy (%)^a
5	4.64 ± 0.23	4.8	-7.2	4.56 ± 0.30	6.6	−8.8
8	8.22 ± 0.44	5.3	2.8	8.13 ± 0.44	5.4	1.6
80	85.7 ± 4.01	4.7	7.1	82.2 ± 3.31	4.0	2.8
800	824 ± 31.2	3.8	3.0	817 ± 19.2	2.4	2.1

Concentration is in 3 significant figures; SD, standard deviation; RSD%, percent relative standard deviation (in one decimal place). ^aExpressed as ((mean measured concentration−nominal concentration)/(nominal concentration)) × 100 (in one decimal place).