Determination of Isosorbide-5-Mononitrate in Human Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Bioequivalence Study

Table 5

Precision and accuracy of 5-ISMN in hyperlipidemia and hemolysis matrices (n = 6).

Matrix	Nominal con. (ng/mL)	Measured con. (mean ± SD, ng/mL)	Precision (RSD %)	Accuracy (%)^a
300 mg/dL hyperlipidemic matrix	8	7.79 ± 0.37	4.7	−2.6
	80	82.5 ± 3.89	4.7	3.1
	800	831 ± 23.18	2.8	3.8
2% hemolysis matrix	8	7.91 ± 0.46	5.8	−1.1
	80	80.8 ± 2.74	3.4	1.0
	800	852 ± 14.15	1.7	6.5

Concentration is in 3 significant figures; SD, standard deviation; RSD%, percent relative standard deviation. ^aExpressed as ((mean measured concentration−nominal concentration)/(nominal concentration)) × 100 (in one decimal place).