A Simple and Rapid LC-MS/MS Method for Quantification of Total Daidzein, Genistein, and Equol in Human Urine

Figure 1

Daidzein, genistein, and equol sulfate metabolites after hydrolysis using a glucuronidase/sulfatase mixture (200/20 U) for 2 h at pH 5 and 37°C. LC-MS/MS extracted ion chromatograms of (a) 333/253 (daidzein sulfate), (b) 349/269 (genistein sulfate), and (c) 321/241 (equol sulfate).

(a)

(b)

(c)