Simultaneous Determination of Malachite Green, Chloramphenicols, Sulfonamides, and Fluoroquinolones Residues in Fish by Liquid Chromatography-Mass Spectrometry

Table 1

Transitions and optimal conditions used for MS/MS analysis.

Analyte	Retention time (min)	Precursor ion(m/z)	Product ion(m/z)	Declustering potential (V)	Collision energy (eV)
Fleroxacin	7.73	370	269/326	90	26/19
Ofloxacin	7.75	362	261/318	85	27/18
Norfloxacin	7.75	320.3	233.4/276.3	85	26/26
Enoxacin	6.79	321	232/303	85	34/21
Ciprofloxacin	7.33	332.2	245.3/288.3	85	35/25
Enrofloxacin	8.07	360.2	245.2/316.3	90	39/27
Lomefloxacin	7.66	352	265/308	85	22/16
Danofloxacin	7.86	358	283/340	85	22/22
Orbifloxacin	7.96	396	295/352	85	24/17
Difloxacin	8.07	400	299/356	85	28/19
Sarafloxacin	8.05	386	299/342	85	26/18
Sparfloxacin	9.04	393	292/349	85	25/19
Oxolinic acid	9.72	262	216/244	85	29/18
Flumequine	10.07	262	202/244	85	32/18
Pefloxacin	7.20	334	290/316	85	18/20
Nalidixic acid	10.01	233.3	187.4/215.2	85	36/24
Sulfisoxazole	9.49	268	108/156	85	22/13
Sulfisomidine	7.05	279	186/156	85	17/19
Sulfathiazole	4.65	256	108/156	85	22/16
Sulfapyridine	4.98	250	184/156	85	18/16
Sulfamonomethoxine	8.44	281	215/156	85	17/17
Fleroxacin	7.49	281	215/156	85	17/17
Ofloxacin	6.90	254	108/156	85	22/16
Norfloxacin	7.21	271	107/156	85	30/14
Enoxacin	3.26	279	186/156	85	17/19
Ciprofloxacin	7.02	281	215/156	85	17/17
Enrofloxacin	5.50	265	172/156	85	17/17
Lomefloxacin	0.94	215	108/156	85	22/13
Danofloxacin	9.78	311	108/156	85	29/19
Orbifloxacin	9.28	311	108/156	85	29/19
Difloxacin	4.59	251	108/156	85	25/16
Sarafloxacin	8.34	285	108/156	85	24/15
Sparfloxacin	8.98	301	108/156	85	25/17
Oxolinic acid	8.84	277.3	107.7/156	80	20/12
Flumequine	8.90	285	108/156	80	37/24
Pefloxacin	11.05	315	160/158	80	23/25
Nalidixic acid	2.93	215	108/156	80	20/10
Sulfisoxazole	9.15	268	112.8/155.8	80	20/15
Sulfisomidine	9.90	329.3	208.1/313.1	110	49/49
Sulfathiazole	11.84	331.3	239/316	110	42/33
Sulfapyridine	10.55	321	257/152	-60	−16/−26
Sulfamonomethoxine	10.29	356	185/336	-60	−14/−27
Sulfadoxine-D3	9.29	314	156	85	17
Sulfadimethoxine-D6	9.78	317	156	85	20
Norfloxacin-D5	7.36	325.3	307.2	80	30
Ciprofloxacin-D8	7.57	340.3	322.3	85	29
Enrofloxacin-D5	8.24	365.3	321.3	94	28
Malachite green-D5	9.92	334.3	318.2	120	52
Leucomalachite green-D6	11.79	337.3	322.3	110	31
Chloramphenicol-D5	10.54	326	157	−60	−26

Transitions for quantification.