Simultaneous Determination of Malachite Green, Chloramphenicols, Sulfonamides, and Fluoroquinolones Residues in Fish by Liquid Chromatography-Mass Spectrometry

Table 5

Parameters of UPLC-MS/MS for the 42 compounds in fish sample.

Analyte	Spiked levels	Recoveries R (%)	Internal standard	LOD μg·kg^−1	LOQ μg·kg^−1	Intraday RSD(%) μg·kg^−1	Inter-day RSD(%) μg·kg^−1
Fleroxacin	0.5/1/4	71.2/73.4/75.5	Enrofloxacin-D5	0.1	0.4	3.6	4.1
Sulfamethoxazole	0.5/1/4	83.6/81.5/92.4	Sulfadimethoxine-D6	0.1	0.4	1.4	2.2
Ofloxacin	0.5/1/4	77.6/74.8/81.2	Enrofloxacin-D5	0.1	0.4	1.2	1.1
Sulfamethizol	0.5/1/4	74.4/78.8/82.5	Sulfadimethoxine-D6	0.1	0.4	2.7	3.3
Norfloxacin	1/2/10	101/102/106	Norfloxacin-D5	0.25	1.0	4.4	3.8
Sulfamethazine	0.5/1/4	92.2/95.4/96.7	Sulfadimethoxine-D6	0.1	0.4	1.5	1.7
Enoxacin	0.5/1/4	75.5/73.6/78.9	Enrofloxacin-D5	0.1	0.4	1.8	2.3
Sulfameter	0.5/1/4	88.1/90.3/94.6	Sulfadimethoxine-D6	0.1	0.4	1.8	2.5
Ciprofloxacin	1/2/10	82.1/85.4/88.6	Ciprofloxacin-D8	0.25	1.0	2.4	2.6
Sulfamerazine	0.5/1/4	95.5/94.6/1003	Sulfadimethoxine-D6	0.1	0.4	1.3	3.4
Enrofloxacin	0.5/1/4	101/104/107	Enrofloxacin-D5	0.1	0.4	2.7	1.2
Sulfaguanidine	0.5/1/4	71.6/72.4/76	Sulfadimethoxine-D6	0.1	0.4	1.3	2.3
Lomefloxacin	1/2/10	75.5/75/77.3	Enrofloxacin-D5	0.25	1.0	3.4	3.7
Sulfadoxine	0.5/1/4	105/107/104	Sulfadoxine-D3	0.1	0.4	2.1	4.3
Danofloxacin	0.5/1/4	76.6/76.7/81	Enrofloxacin-D5	0.1	0.4	1.6	1.9
Sulfadimethoxine	0.5/1/4	83.2/87.2/91.8	Sulfadimethoxine-D6	0.1	0.4	1.4	2.7
Orbifloxacin	0.5/1/4	83.6/83.8/87	Enrofloxacin-D5	0.1	0.4	1.1	1.8
Sulfadiazine	0.5/1/4	92.6/93.2/103	Sulfadoxine-D3	0.1	0.4	2.3	3.8
Difloxacin	0.5/1/4	72.2/77.5/77	Enrofloxacin-D5	0.1	0.4	1.5	1.4
Sulfachloropyridazine	0.5/1/4	82.6/82.7/84.2	Sulfadimethoxine-D6	0.1	0.4	2.2	4.5
Sarafloxacin	1/2/10	84.9/77.5/83.4	Sulfadimethoxine-D6	0.25	1.0	1.4	2.2
Sulfachinoxalin	0.5/1/4	67.2/71/71.5	Sulfadimethoxine-D6	0.1	0.4	2.6	4.1
Sparfloxacin	1/2/10	84.4/86.5/78.3	Enrofloxacin-D5	0.25	1.0	1.8	2.5
Sulfabenzamide	0.5/1/4	71.6/72.4/75.8	Sulfadimethoxine-D6	0.1	0.4	3.1	5.6
Oxolinic acid	0.5/1/4	69.2/71.4/73.3	Enrofloxacin-D5	0.1	0.4	2.7	3.2
Sulfachloropyrazine	2001/2/10	73.7/81/76.3	Sulfadimethoxine-D6	0.25	1.0	2.4	3.5
Flumequine	0.5/1/4	68.2/72.2/77.5	Enrofloxacin-D5	0.1	0.4	1.7	1.6
Sulfaphenazole	0.5/1/4	70.4/72.3/73.8	Sulfadimethoxine-D6	0.1	0.4	3.5	2.8
Pefloxacin	0.5/1/4	80.2/79/82.4	Enrofloxacin-D5	0.1	0.4	3.5	3.8
Sulfacetamide	0.5/1/4	74.2/75/80	Sulfadimethoxine-D6	0.1	0.4	3.7	3.5
Nalidixic acid	0.5/1/4	69.6/69.8/73.5	Enrofloxacin-D5	0.1	0.4	5.2	6.7
Sulfamoxol	0.5/1/4	83.6/85.2/87.8	Sulfadimethoxine-D6	0.1	0.4	3.3	4.6
Sulfisoxazole	1/2/10	93.5/93/89.8	Sulfadimethoxine-D6	0.25	1.0	1.1	2.4
Sulfamethoxypyridazine	0.5/1/4	78/81.9/86.2	Sulfadimethoxine-D6	0.1	0.4	1.7	1.8
Sulfisomidine	1/2/10	73.4/77/82.2	Sulfadimethoxine-D6	0.25	1.0	2.5	2.2
Sulfathiazole	0.5/1/4	83.8/80.7/81.5	Sulfadimethoxine-D6	0.1	0.4	2.6	3.3
Sulfapyridine	0.5/1/4	67/69.8/72.5	Sulfadimethoxine-D6	0.1	0.4	4.4	7.1
Sulfamonomethoxine	0.5/1/4	87/89.4/92	Sulfadimethoxine-D6	0.1	0.4	1.2	1.9
Malachite green	0.5/1/4	91/93.3/92.2	Malachite green-D5	0.1	0.4	2.6	1.6
Leucomalachite green	0.5/1/4	86.5/91.9/92.8	Leucomalachite green-D6	0.1	0.4	1.9	4.2
Chloramphenicol	0.8/4.0/20	91.6/95.2/106	Chloramphenicol-D5	0.15	0.5	1.6	3.8
Florfenicol	0.8/4.0/20	87.9/92.3/98	Chloramphenicol-D5	0.15	0.5	2.1	1.7