Research Article
Simultaneous Determination of 34 Amino Acids in Tumor Tissues from Colorectal Cancer Patients Based on the Targeted UHPLC-MS/MS Method
Table 2
The optimized MRM parameters of 34 amino acids and 3 ISs (ESI positive).
| Analyte | Molecular weight | Precursor ion | Product ion | Fragmentor (V) | Collision energy (eV) |
| Gly | 75.07 | 76 | 30 | 50 | 13 | Ala | 89.09 | 90 | 44 | 50 | 8 | Ser | 105.09 | 106 | 60 | 65 | 9 | Val | 117.15 | 118 | 72 | 60 | 7 | Thr | 119.12 | 120 | 74 | 65 | 9 | Leu | 131.17 | 132 | 86 | 65 | 7 | Ile | 131.17 | 132 | 86 | 65 | 7 | Asp | 133.10 | 134 | 74 | 65 | 12 | Lys | 146.19 | 147 | 84 | 70 | 11 | Gln | 146.14 | 147 | 84 | 65 | 12 | Glu | 147.13 | 148 | 84 | 70 | 12 | Phe | 165.19 | 166 | 120 | 65 | 10 | Arg | 174.20 | 175 | 70 | 90 | 16 | Tyr | 181.19 | 182 | 136 | 70 | 11 | Pro | 115.13 | 116 | 70 | 70 | 15 | Asn | 132.12 | 133 | 74 | 60 | 11 | Met | 149.21 | 150 | 56 | 65 | 13 | Trp | 204.23 | 205 | 188 | 70 | 5 | Cys | 121.16 | 122 | 59 | 60 | 21 | His | 155.15 | 156 | 110 | 80 | 13 | Cit | 175.19 | 176 | 159 | 70 | 7 | ADMA | 202.25 | 203 | 46 | 90 | 16 | Cyss | 240.30 | 241 | 152 | 80 | 12 | Sar | 89.09 | 90 | 44 | 55 | 10 | Apa | 89.09 | 90 | 30 | 60 | 12 | Amp | 103.12 | 104 | 30 | 60 | 11 | Aba | 103.12 | 104 | 87 | 65 | 9 | Opr | 129.11 | 130 | 84 | 75 | 12 | Hpr | 131.13 | 132 | 86 | 75 | 13 | Orn | 132.16 | 133 | 70 | 65 | 10 | Ahd | 161.16 | 162 | 98 | 65 | 11 | Hia | 179.17 | 180 | 105 | 65 | 6 | SDMA | 202.25 | 203 | 172 | 90 | 14 | Kyn | 208.21 | 209 | 192 | 70 | 7 | Ala-d4 | 93.12 | 94 | 48 | 50 | 9 | Met-d3 | 152.23 | 153 | 56 | 65 | 13 | Phe-d5 | 170.22 | 171 | 125 | 70 | 11 |
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