Journal of Analytical Methods in Chemistry

Research Article

Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells

Table 1

Constituents identified from YZG by UPLC-ESI-Q-TOF-MS.


No.	Rt	Formula	[M + HCOO]⁻ (m/z)	[M-H]⁻ (m/z)	Fragment ions (m/z)	CMP	Compound name	Reference

1	3.42	C₄₂H₇₂O₁₅	861.4843	815.4784	653.4254, 491.2235, 463.0880	√	Notoginsenoside M	[23]
2	3.62	C₂₇H₃₀O₁₆		609.1816	300.0555	√	Rutin	[24]
3	3.72	C₈H₈O₅		183.0308	124.0211	√	Methyl gallate	[25]
4	4.22	C₄₈H₈₂O₁₉	1007.5439	961.5380	997.5126, 799.4825, 637.4305, 475.1966	√	Notoginsenoside R3/R6/20-O-Glucoginsenoside Rf	[23]
5	4.28	C₄₀H₇₀O₈	723.5082	677.5007	740.4865, 475.1963, 417.1550, 284.0329		Unknown
6	4.47	C₄₃H₈₃O₁₂	836.5914	790.5905	853.5441		Unknown
7	4.55	C₄₈H₈₂O₁₉	1007.5439	961.5406	997.5145, 799.4846, 637.4318, 475.3790	√	Notoginsenoside R3/R6/20-O-Glucoginsenoside Rf	[23]
8	4.57	C₃₂H₃₈O₁₅	707.2444	661.2957	1323.5775, 724.2191, 499.1783		Baohuoside A	∆
9	4.61	C₄₇H₈₀O₁₈	977.5385	931.5317	799.4850, 637.4321, 475.3785	√	Notoginsenoside R1	[26]
10	4.81	C₄₈H₈₂O₁₈	991.5449	945.5477	799.4883, 637.4339, 619.4214, 475.3792	√	Ginsenoside Re	[27]
11	4.85	C₄₂H₇₂O₁₄	845.4936	799.4833	637.4339, 475.3792	√	Ginsenoside Rg1	[27]
12	5.24	C₃₉H₅₀O₂₀	883.2853	837.2800	675.2280, 529.2005, 513.1791, 367.1183		Epimedin A1	∆
13	5.29	C₁₅H₁₀O₇		301.0683	273.0429, 245.0488, 229.0532, 179.0065, 151.0179, 121.0346		Quercetin	∆
14	5.31	C₃₈H₄₈O₁₉		807.2960	645.2173, 513.1818, 366.1090		Epimedin B	∆
15	5.38	C₃₈H₅₀O₁₉	867.2896	821.2642	659.2647, 513.1761, 366.1201, 351.0912, 323.0849, 151.0094, 106.6611		Epimedin C	∆
16	5.40	C₃₈H₅₀O₁₉	867.3390	821.2833	659.2668, 366.1226		Epimedin A	∆
17	5.53	C₂₇H₃₀O₁₀		513.1833	367.1188, 351.0876, 323.0917, 151.0109, 145.0312		Isomer⁽⁴⁷⁾	∆
18	5.53	C₃₃H₄₀O₁₅	721.2323	675.2302	529.1712, 513.1846, 367.1266		Icariin	∆
19	5.64	C₄₈H₈₂O₁₉	1007.5430	961.5372	799.4822, 637.3738	√	Notoginsenoside N/R6	[28]
20	5.67	C₅₄H₉₂O₂₃	1153.6027	1107.5963	945.5418, 799.4827, 637.4309, 475.1394,		Ginsenoside Re8	[29]
21	5.90	C₅₉H₁₀₀O₂₇	1285.6840	1239.6516	1107.5920, 945.5372, 783.4835, 621.4189, 459.3343	√	Notoginsenoside Fa	∆
22	6.09	C₄₂H₇₂O₁₄	845.4875	799.4935	637.4294, 619.5074, 475.3772	√	Ginsenoside Rf	[28]
23	6.11	C₅₄H₉₂O₂₃	1153.6823	1107.6227	945.5391, 783.4855, 621.4235, 459.3650	√	Ginsenoside Rb1	[30]
24	6.21	C₅₂H₈₆O₂₁	1091.5645	1045.7167	913.5291, 751.4626, 605.4065, 473.3609,	√	Gypenoside XLIX	∆
25	6.26	C₅₃H₉₀O₂₂	1123.5693	1077.6210	945.5375, 783.4875	√	Ginsenoside Rc	[30]
26	6.28	C₄₁H₇₀O₁₃	815.4929	769.4709	637.4373, 619.4420, 475.3855	√	20(S)-Notoginsenoside R2	∆
27	6.33	C₄₁H₇₀O₁₃	815.4812	769.4928	637.4339, 475.3815	√	Notoginsenoside R2/F3/F5	[28]
28	6.40	C₆₃H₁₀₆O₃₀		1341.5354	1209.6248, 1077.5825, 945.5411, 783.4882, 621.4359, 459.3843	√	Notoginsenoside Q	[28]
29	6.41	C₄₈H₇₆O₁₉		955.4913	793.4395, 569.3852	√	Ginsenoside rRo	[31]
30	6.42	C₅₃H₉₀O₂₂	1123.5911	1077.5951	945.5395, 783.4940, 621.4150, 459.3434	√	Ginsenoside Rb2	[28]
31	6.44	C₅₃H₉₀O₂₂	1123.5911	1077.5911	945.5429, 783.4907, 459.3839	√	Ginsenoside Rb3	[28]
32	6.46	C₄₂H₇₂O₁₃	829.5134	783.5062	637.4319, 619.4190, 475.3806	√	20(S)-Ginsenoside Rg2	[30]
33	6.50	C₄₁H₇₀O₁₃	815.4929	769.4709	637.4373, 619.4420, 475.3855		20(R)-Notoginsenoside R2	∆
34	6.56	C₃₆H₆₂O₉	683.4399	637.4317	475.3788		20(S)-Ginsenoside Rh1/20(R)-Ginsenoside Rh1	[28]
35	6.56	C₄₂H₇₂O₁₃	829.4958	783.4899	637.4317, 475.3788	√	20(R)-Ginsenoside Rg2	[30]
36	6.59	C₅₆H₉₄O₂₄	1195.6116	1149.6077	1107.5959, 945.5424, 783.4896, 637.4297, 475.3786	√	Yesanchinoside F	[32]
37	6.85	C₄₈H₈₂O₁₈	991.5511	945.5786	783.4921, 621.4363, 459.3667	√	Ginsenoside Rd	[28]
38	6.98	C₅₁H₁₀₀O₃₀	1237.6125	1191.6177	1029.5610, 915.5285, 637.4301, 475.3689		Unknown
39	7.21	C₅₂H₈₆O₂₁	943.5059	897.5676	765.4807, 751.4272, 681.4173, 619.3864, 487.3429	√	Gypenoside A	∆
40	7.26	C₅₅H₉₂O₂₃	1165.6019	1119.5986	783.4898, 621.4372, 459.3844	√	Ginsenoside Rs2	[28]
41	7.44	C₂₇H₃₀O₁₁	575.3054	529.1713	1105.6187, 1059.6130, 367.1176		Icariside I
42	7.49	C₄₇H₈₀O₁₇	961.5392	915.5334	783.4893, 621.4371		Notoginsenoside Fe/gynosaponin I/vinaginsenoside R16/R17	[28]
43	7.61	C₆₀H₁₁₆O₃₆	1457.7361	1411.7128	1265.6560, 1133.6172, 987.5623, 841.4951, 475.3750		Unknown
44	7.62	C₄₈H₈₂O₁₈	991.5460	945.5498	799.4841, 637.4338, 619.4188, 475.3750		Unknown
45	7.63	C₅₁H₈₆O₂₁	1033.6028	987.5575	945.5498, 927.5349, 841.4951	√	Pseudoginsenoside Rc1	[28]
46	7.81	C₅₅H₉₂O₂₃	1165.6125	1119.6310	1077.5851, 1059.5736, 945.5401, 927.5275, 765.4456, 621.3965, 459.2525	√	Ginsenoside Rs1	[28]
47	7.82	C₂₇H₃₀O₁₀		513.1867	366.1149, 351.0916, 323.0886, 151.0092, 132.0236		Baohuoside I	∆
48	7.82	C₅₀H₉₈O₂₉	1207.6355	1161.6063	1175.6207, 1161.6063		Unknown
49	7.85	C₄₁H₇₀O₁₄	831.4783	785.4698	653.4258		Notoginsenoside Rw2	[33]
50	7.89	C₅₄H₈₆O₂₄	1163.6464	1117.6188	1057.5961, 971.5587, 929.5478		Hemsloside G2/ginsenoside ROA	[34]
51	8.05	C₄₂H₇₀O₁₂	811.4849	765.4782	619.4163, 457.2605		Ginsenoside Rg6	∆
52	8.14	C₅₂H₁₀₄O₃₀	1253.6549	1207.6088	1207.6088, 1075.5676, 943.5283		Unknown
53	8.16	C₄₂H₇₂O₁₃	829.4985	783.4911	637.4318, 475.3790	√	Ginsenoside Fc	[28]
54	8.17	C₅₁H₈₆O₂₁	1033.5596	987.5541	945.5425 927.5313, 783.4911, 765.4796, 621.4370, 459.3835	√	Quinquenoside III	[28]
55	8.18	C₄₂H₇₂O₁₄	845.4895	799.4831	637.4308, 619.4199, 475.3775		Isomer⁽²²⁾	[28]
56	8.42	C₄₈H₈₂O₁₆	959.5573	913.5543	767.4940, 621.4166, 475.3465, 146.9997		Gynosaponin II	[35]
57	8.50	C₅₆H₉₄O₂₄		1149.6082	1107.7029, 945.5416, 927.5334, 783.4859, 765.3539, 621.4349, 459.3838	√	Quinquenoside R1	[28]
58	8.72	C₄₂H₇₂O₁₃	829.4963	783.4920	767.4591, 621.4376, 459.3846	√	20(S)-Ginsenoside Rg3	[23]
59	8.77	C₄₂H₇₂O₁₃	829.4974	783.4915	765.4796, 621.4368, 459.3842	√	Isomer⁽⁵⁸⁾	[23]
60	8.86	C₄₂H₇₂O₁₃	829.5020	783.4990	621.4348, 459.3762, 161.0500	√	20(R)-Ginsenoside Rg3	[23]
61	9.39	C₃₆H₆₂O₉	683.4375	637.4319	475.3790		20(S)-Ginsenoside Rh1/20(R)-Ginsenoside Rh1	[28]
62	9.54	C₄₇H₈₀O₁₈	977.4802	931.5010	799.4818, 769.4988, 751.4977, 637.3975		Isomer⁽⁹⁾	[28]
63	9.59	C₄₉H₈₀O₁₈	1001.5699	955.5613	913.5525, 767.4947		Gylongiposide I	[36]
64	9.60	C₄₇H₈₀O₁₇	961.5573	915.5556	783.4860, 621.4335, 459.3809		Notoginsenoside Fe/gynosaponin I/vinaginsenoside R16/R17	[28]
65	10.22	C₄₂H₇₀O₁₂	811.4861	765.4803	619.4220, 603.4269, 457.2275		Ginsenoside Rg6	[28]
66	10.26	C₄₂H₇₂O₁₃	829.4875	783.4943	621.4368, 459.3837	√	Ginsenoside F2	[28]
67	10.36	C₄₂H₇₀O₁₂	811.4857	765.4805	619.4229, 603.4269, 457.2340		Ginsenoside F4	[28]
68	10.38	C₄₂H₇₀O₁₂	811.4849	765.4792	603.3373		Ginsenoside Rg5	[28]
69	10.40	C₄₂H₇₀O₁₂	811.4850	765.4794	603.3384, 483.2727		Ginsenoside Rk1	[28]
70	15.05	C₄₇H₈₀O₁₇	961.6223	915.5983	978.5970, 783.5145, 621.3875, 459.3448	√	Notoginsenoside Fe/gynosaponin I/vinaginsenoside R16/R17	[28]

Abbreviations: Rt, retention time; CMP, cynomolgus monkeys plasma; Ref., references. √: compound detected; : compound not detected. isomer^(N°^.): numbers in parentheses represent the peak corresponding to isomers; ∆: the data are consistent with the standard substance.