Research Article
Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples
Table 1
Preparation of FA standards and blood samples.
| Order | Mixing volume (μL) | Concentration (ng μL−1) |
| | Primary standarda | Distilled water | (ng μL−1) | A. First working standards (first WSs) | 1 | 2 | 1,998 | 403 | 2 | 10 | 1,990 | 2,017 | 3 | 20 | 1,980 | 4,033 | 4 | 50 | 1,950 | 10,083 | 5 | 100 | 1,900 | 20,165 |
| | First point of first WSs | Distilled water | (ng μL−1) | B. Final working standards (F-WSs) | 1 | 2 | 1,998 | 0.403 | 2 | 10 | 1,990 | 2.02 | 3 | 20 | 1,980 | 4.03 | 4 | 50 | 1,950 | 10.1 | 5 | 100 | 1,900 | 20.2 | 6 | 200 | 1,800 | 40.3 | 7 | 1,000 | 1,000 | 202 |
| C. Blood samples | Experimental animal | Yucatan minipig | | | Sex | Male | | | Age | 10 weeks | | | Number of animals | Three | | | Blood sampling point | Ear vein | | | Blood sampling method | Intravenous (IV) | | | Sampling volume | Each 2 mL | | | Blood sample codes | Blood samples A, B, and C | | |
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aThe purchased primary standard of formaldehyde was 37% pure (403,300 ng μL−1).
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