Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples

Table 1

Preparation of FA standards and blood samples.

Order	Mixing volume (μL)		Concentration (ng μL^−1)
	Primary standard^a	Distilled water	(ng μL^−1)
A. First working standards (first WSs)
1	2	1,998	403
2	10	1,990	2,017
3	20	1,980	4,033
4	50	1,950	10,083
5	100	1,900	20,165
	First point of first WSs	Distilled water	(ng μL^−1)
B. Final working standards (F-WSs)
1	2	1,998	0.403
2	10	1,990	2.02
3	20	1,980	4.03
4	50	1,950	10.1
5	100	1,900	20.2
6	200	1,800	40.3
7	1,000	1,000	202
C. Blood samples
Experimental animal	Yucatan minipig
Sex	Male
Age	10 weeks
Number of animals	Three
Blood sampling point	Ear vein
Blood sampling method	Intravenous (IV)
Sampling volume	Each 2 mL
Blood sample codes	Blood samples A, B, and C

^aThe purchased primary standard of formaldehyde was 37% pure (403,300 ng μL⁻¹).