Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples

Table 2

Experimental scheme for the development and validation of the pretreatment method for FA analysis in blood samples.

Experimental codes	Contents	Pretreatment method	Target material^a	Concentration (ng μL^−1)		Number of calibration points	Dilution factor in pretreatment procedures
				Standards^b	Analytical^c
Exp 1a	Analysis of FA in standards	Pretreatment B	20 μL first WSs and F-WSs	0.40 to 20,165	0.004 to 202	9 points	100
Exp 1b			200 μL F-WSs	0.40 to 202	0.012 to 5.761	4 points	35
Exp 2	Blood sample analysis	Pretreatments A and B	200 μL blood sample	NA	NA	NA
Exp 3	Spiking sample analysis (blood sample + standards)		200 μL blood sample + 2 μL first WSs	4,033 to 20,165 for first WSs	1.22 to 6.09 for first WSs	3 points	-

^aWS: working standard; F-WS: final working standard. ^bExp 1a: 0.40, 2.02, 10.08, 20.17, 403, 2,017, 4,033, 10,083, and 20,165 ng μL^–1; Exp 1b: 0.40, 4.03, 40.33, and 201.65 ng μL^–1; Exp 3 : 4,033, 10,083, and 20,165 ng μL^–1. ^cExp 1a: 0.004, 0.020, 0.1008, 0.2017, 4.03, 20.17, 40.33, 100.83, and 201.65 ng μL^–1; Exp 1b: 0.012, 0.115, 1.152, and 5.761 ng μL^–1; Exp 3 : 1.22, 3.05, and 6.09 ng μL^–1. NA: not available.