Simultaneous Determination of Five Components of Chaihu-Shugan-San in Beagle Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study after a Single Dose of Chaihu-Shugan-San

Table 5

The typical regression equations, correlation coefficient, and LLOQ in beagle plasma determined by HPLC-MS/MS.

Analyte	Linear range (ng/ml)	Regression equation	R^2	LLOQ (ng/mL)
Neohesperidin	0.5–100	y = 1.79 × 10^−3x + 4.76 × 10^−3	0.9993	0.5
Hesperidin	0.5–100	y = 1.40 × 10^−2x + 2.32 × 10^−2	0.9953	0.5
Naringin	1–200	y = 5.09 × 10^−3x + 8.59 × 10^−3	0.9980	1
Paeoniflorin	1–200	y = 7.54 × 10^−3x + 2.85 × 10^−2	0.9937	1
Liquiritin	1–200	y = 1.57 × 10^−2x + 4.73 × 10^−2	0.9923	1