Simultaneous Determination of Five Components of Chaihu-Shugan-San in Beagle Plasma by HPLC-MS/MS and Its Application to a Pharmacokinetic Study after a Single Dose of Chaihu-Shugan-San

Table 6

The precision, accuracy, recovery, and matrix effects of five analytes in beagle plasma.

Analytes	Added (ng/mL)	Intraday			Interday			Recovery (%)	ME (%)
		Detected (ng/mL)	RSD (%)	RE (%)	Detected (ng/mL)	RSD (%)	RE (%)
Neohesperidin	1	0.99 ± 0.05	5.32	−1.50	0.98 ± 0.02	2.35	−1.56	89.97 ± 8.02	99.90 ± 3.62
	25	25.28 ± 1.52	6.02	1.13	25.40 ± 0.85	3.35	1.58	85.42 ± 3.74	102.79 ± 5.37
	75	74.92 ± 3.29	4.39	−0.11	75.47 ± 2.47	3.27	0.63	87.21 ± 5.95	100.66 ± 7.42
Hesperidin	1	0.97 ± 0.03	3.45	−3.00	0.99 ± 0.04	3.90	−1.28	89.09 ± 7.62	98.27 ± 4.14
	25	24.58 ± 1.37	5.59	−1.67	24.89 ± 0.35	1.42	−0.43	90.70 ± 6.24	99.41 ± 7.38
	75	76.28 ± 3.40	4.45	1.70	74.50 ± 2.64	3.55	−0.67	89.42 ± 4.52	100.89 ± 5.56
Naringin	2.5	2.47 ± 0.13	5.29	−1.13	2.49 ± 0.04	1.55	−0.51	88.57 ± 5.90	101.86 ± 7.47
	50	50.67 ± 3.85	7.61	1.33	51.15 ± 2.10	4.11	2.29	86.69 ± 6.47	103.53 ± 5.87
	150	150.65 ± 8.51	5.65	0.44	149.39 ± 2.27	1.52	−0.41	90.85 ± 5.51	103.45 ± 3.98
Paeoniflorin	2.5	2.39 ± 0.17	6.98	−4.53	2.37 ± 0.06	2.68	−5.11	89.37 ± 4.99	103.91 ± 6.70
	50	48.27 ± 3.38	7.01	−3.46	48.44 ± 0.93	1.92	−3.12	87.18 ± 7.05	94.52 ± 5.97
	150	147.59 ± 9.13	6.18	−1.61	151.61 ± 2.90	1.91	1.08	89.27 ± 5.25	100.68 ± 4.65
Liquiritin	2.5	2.33 ± 0.18	7.51	−6.67	2.49 ± 0.04	1.66	−0.29	90.12 ± 6.41	102.03 ± 8.05
	50	49.29 ± 4.34	8.80	−1.41	51.16 ± 1.63	3.19	−1.60	89.82 ± 6.38	100.62 ± 7.64
	150	147.60 ± 5.78	3.91	−1.60	153.97 ± 6.00	3.90	2.64	87.41 ± 5.81	99.29 ± 6.46