Journal of Analytical Methods in Chemistry

Research Article

Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 2

Calibration curves, correlation coefficient, linear range, and LLOQ of 12 compounds.


Compound	Tissue	Linear regression equation	Correlation coefficient (r)	Linear range (ng/mL)	LLOQ (ng/mL)

Psoralen	Heart	Y = 7.77 × 10⁻³X + 2.23 × 10⁻²	1.0000	1–1000	1.0
	Liver	Y = 3.60 × 10⁻³X + 1.03 × 10⁻¹	1.0000	5–1000	5.0
	Spleen	Y = 5.71 × 10⁻³X + 2.92 × 10⁻²	0.9995	5–1000	5.0
	Lung	Y = 2.44 × 10⁻³X + 3.33 × 10⁻²	0.9980	5–1000	5.0
	Kidney	Y = 3.44 × 10⁻³X + 1.21 × 10⁻¹	0.9996	2–1000	2.0
	Uterus	Y = 8.62 × 10⁻³X + 8.86 × 10⁻³	1.0000	1–1000	1.0
	Ovary	Y = 6.53 × 10⁻³X + 1.79 × 10⁻²	1.0000	5–1000	5.0
	Testes	Y = 5.04 × 10⁻³X + 4.89 × 10⁻²	1.0000	1–1000	1.0

Isopsoralen	Heart	Y = 8.57 × 10⁻³X + 3.61 × 10⁻²	1.0000	1–1000	1.0
	Liver	Y = 1.65 × 10⁻³X + 1.74 × 10⁻¹	0.9986	5–1000	5.0
	Spleen	Y = 4.55 × 10⁻³X + 3.50 × 10⁻²	0.9981	5–1000	5.0
	Lung	Y = 1.48 × 10⁻³X + 5.62 × 10⁻²	0.9970	5–1000	5.0
	Kidney	Y = 2.34 × 10⁻³X + 1.77 × 10⁻¹	0.9994	2–1000	2.0
	Uterus	Y = 6.23 × 10⁻³X + 2.86 × 10⁻²	1.0000	1–500	1.0
	Ovary	Y = 6.12 × 10⁻³X + 5.19 × 10⁻²	1.0000	5–1000	5.0
	Testes	Y = 4.27 × 10⁻³X + 7.24 × 10⁻²	0.9997	5–1000	5.0

Bavachin	Heart	Y = 2.31 × 10⁻²X − 6.13 × 10⁻³	1.0000	0.4–50	0.4
	Liver	Y = 3.17 × 10⁻²X + 8.99 × 10⁻²	0.9977	1–500	1.0
	Spleen	Y = 2.61 × 10⁻²X + 3.94 × 10⁻²	1.0000	1–200	1.0
	Lung	Y = 4.07 × 10⁻²X + 2.90 × 10⁻²	0.9976	0.5–200	0.5
	Kidney	Y = 2.19 × 10⁻²X + 6.66 × 10⁻³	0.9999	1–500	1.0
	Uterus	Y = 2.11 × 10⁻²X − 4.30 × 10⁻²	0.9997	1–200	1.0
	Ovary	Y = 3.70 × 10⁻²X − 1.14 × 10⁻²	1.0000	1–50	1.0
	Testes	Y = 2.14 × 10⁻²X − 2.71 × 10⁻²	0.9897	1–50	1.0

Isobavachin	Heart	Y = 4.07 × 10⁻²X + 8.55 × 10⁻³	0.9996	0.3–50	0.3
	Liver	Y = 5.35 × 10⁻²X + 5.28 × 10⁻²	0.9999	1–250	1.0
	Spleen	Y = 3.82 × 10⁻²X + 8.21 × 10⁻²	0.9995	0.5–100	0.5
	Lung	Y = 5.94 × 10⁻²X + 3.32 × 10⁻²	0.9976	0.5–100	0.5
	Kidney	Y = 4.62 × 10⁻²X + 1.85 × 10⁻²	1.0000	0.1–250	0.1
	Uterus	Y = 3.93 × 10⁻²X + 3.14 × 10⁻³	1.0000	0.1–50	0.1
	Ovary	Y = 7.17 × 10⁻²X − 7.18 × 10⁻²	0.9999	1–50	1.0
	Testes	Y = 3.94 × 10⁻²X + 7.50 × 10⁻²	0.9887	0.5–50	0.5

Pinoresinol diglucoside	Heart	Y = 6.50 × 10⁻⁴X − 2.98 × 10⁻⁴	1.0000	0.5–100	0.5
	Liver	Y = 8.57 × 10⁻⁴X + 6.87 × 10⁻⁵	0.9999	0.5–250	0.5
	Spleen	Y = 4.46 × 10⁻⁴X + 5.35 × 10⁻⁴	1.0000	0.5–100	0.5
	Lung	Y = 6.95 × 10⁻⁴X + 1.26 × 10⁻³	1.0000	0.5–500	0.5
	Kidney	Y = 3.45 × 10⁻⁴X + 5.16 × 10⁻⁴	0.9998	1–1000	1.0
	Uterus	Y = 6.24 × 10⁻⁴X + 6.35 × 10⁻⁴	0.9996	0.5–100	0.5
	Ovary	Y = 7.81 × 10⁻⁴X − 3.66 × 10⁻⁴	0.9997	0.5–100	0.5
	Testes	Y = 6.90 × 10⁻⁴X + 6.48 × 10⁻⁴	0.9999	0.5–50	0.5

Psoralenoside	Heart	Y = 6.99 × 10⁻⁴X + 2.09 × 10⁻³	0.9993	5–1000	5.0
	Liver	Y = 4.63 × 10⁻⁴X + 1.21 × 10⁻²	0.9996	5–1000	5.0
	Spleen	Y = 5.56 × 10⁻⁴X + 2.73 × 10⁻³	0.9975	5–1000	5.0
	Lung	Y = 9.26 × 10⁻⁴X + 2.96 × 10⁻²	0.9998	1–1000	1.0
	Kidney	Y = 3.61 × 10⁻⁴X + 8.94 × 10⁻³	0.9991	5–2000	5.0
	Uterus	Y = 9.86 × 10⁻⁴X + 3.82 × 10⁻²	0.9994	5–2000	5.0
	Ovary	Y = 6.00 × 10⁻⁴X + 1.93 × 10⁻²	0.9998	5–2000	5.0
	Testes	Y = 5.16 × 10⁻⁴X + 1.57 × 10⁻²	0.9973	5–1000	5.0

Isobavachalcone	Heart	Y = 4.89 × 10⁻²X − 5.05 × 10⁻²	0.9997	1–50	1.0
	Liver	Y = 8.17 × 10⁻²X + 9.05 × 10⁻²	0.9973	1–100	1.0
	Spleen	Y = 4.86 × 10⁻²X + 6.15 × 10⁻²	0.9998	0.5–250	0.5
	Lung	Y = 4.93 × 10⁻²X + 1.59 × 10⁻³	0.9998	0.5–250	0.5
	Kidney	Y = 3.18 × 10⁻²X + 2.75 × 10⁻²	0.9993	1–500	1.0
	Uterus	Y = 3.47 × 10⁻²X − 4.89 × 10⁻²	0.9995	1–50	1.0
	Ovary	Y = 2.29 × 10⁻²X + 1.08 × 10⁻²	0.9999	0.5–50	0.5
	Testes	Y = 2.88 × 10⁻²X + 2.11 × 10⁻²	1.0000	0.5–50	0.5

Bavachalcone	Heart	Y = 2.47 × 10⁻²X − 8.09 × 10⁻³	0.9967	0.3–25	0.3
	Liver	Y = 2.83 × 10⁻²X + 1.88 × 10⁻²	0.9999	0.5–250	0.5
	Spleen	Y = 2.83 × 10⁻²X + 2.61 × 10⁻²	0.9996	0.5–50	0.5
	Lung	Y = 3.05 × 10⁻²X + 3.49 × 10⁻²	0.9997	0.5–100	0.5
	Kidney	Y = 3.01 × 10⁻²X + 2.78 × 10⁻²	0.9992	0.5–100	0.5
	Uterus	Y = 3.25 × 10⁻²X − 2.93 × 10⁻²	0.9998	0.5–250	0.5
	Ovary	Y = 2.17 × 10⁻²X + 1.12 × 10⁻²	1.0000	0.5–50	0.5
	Testes	Y = 2.63 × 10⁻²X − 1.85 × 10⁻²	0.9996	0.5–50	0.5

Geniposidic acid	Heart	Y = 1.11 × 10⁻³X + 4.13 × 10⁻⁴	0.9996	1–500	1.0
	Liver	Y = 6.88 × 10⁻⁴X + 3.58 × 10⁻³	0.9993	1–1000	1.0
	Spleen	Y = 1.31 × 10⁻³X + 1.03 × 10⁻³	0.9990	1–500	1.0
	Lung	Y = 8.89 × 10⁻⁴X + 1.78 × 10⁻³	0.9986	1–1000	1.0
	Kidney	Y = 8.11 × 10⁻⁴ X - 1.72 × 10⁻³	0.9992	1–2000	1.0
	Uterus	Y = 5.08 × 10⁻³X + 1.48 × 10⁻²	0.9995	0.5–500	0.5
	Ovary	Y = 8.04 × 10⁻⁴X + 7.50 × 10⁻⁴	0.9990	1–1000	1.0
	Testes	Y = 5.83 × 10⁻⁴X + 3.28 × 10⁻⁴	0.9956	1–250	1.0

Psoralidin	Heart	Y = 9.87 × 10⁻³X − 2.03 × 10⁻³	0.9999	1–250	1.0
	Liver	Y = 7.80 × 10⁻³X + 1.11 × 10⁻²	0.9997	1–500	1.0
	Spleen	Y = 2.43 × 10⁻²X − 2.60 × 10⁻³	0.9998	1–500	1.0
	Lung	Y = 1.54 × 10⁻²X + 1.70 × 10⁻²	0.9993	1–500	1.0
	Kidney	Y = 6.92 × 10⁻²X + 3.91 × 10⁻³	0.9996	1–1000	1.0
	Uterus	Y = 3.96 × 10⁻²X − 6.48 × 10⁻²	0.9996	1–250	1.0
	Ovary	Y = 2.11 × 10⁻²X + 1.84 × 10⁻²	0.9999	0.5–100	0.5
	Testes	Y = 1.56 × 10⁻²X + 1.59 × 10⁻²	0.9996	0.5–50	0.5

Neobavaisoflavone	Heart	Y = 1.39 × 10⁻²X − 5.15 × 10⁻³	0.9994	0.5–50	0.5
	Liver	Y = 7.88 × 10⁻³X + 5.31 × 10⁻²	0.9991	1–1000	1.0
	Spleen	Y = 2.06 × 10⁻²X + 4.61 × 10⁻²	0.9998	1–500	1.0
	Lung	Y = 1.43 × 10⁻²X + 3.16 × 10⁻²	1.0000	1–300	1.0
	Kidney	Y = 1.26 × 10⁻²X − 4.05 × 10⁻³	0.9994	1–1000	1.0
	Uterus	Y = 6.00 × 10⁻²X − 1.22 × 10⁻¹	0.9997	1–50	1.0
	Ovary	Y = 1.81 × 10⁻²X + 1.16 × 10⁻²	0.9996	1–100	1.0
	Testes	Y = 1.40 × 10⁻²X + 1.36 × 10⁻²	0.9998	1–50	1.0

Bavachinin	Heart	Y = 2.72 × 10⁻³X − 2.72 × 10⁻³	0.9999	1–100	1.0
	Liver	Y = 1.48 × 10⁻³X + 3.93 × 10⁻³	0.9998	1–1000	1.0
	Spleen	Y = 7.85 × 10⁻³X − 2.00 × 10⁻²	0.9995	1–1000	1.0
	Lung	Y = 4.39 × 10⁻³X − 7.75 × 10⁻⁴	0.9994	1–500	1.0
	Kidney	Y = 3.51 × 10⁻³X − 3.14 × 10⁻³	0.9992	1–500	1.0
	Uterus	Y = 1.21 × 10⁻²X − 1.96 × 10⁻²	0.9997	1–50	1.0
	Ovary	Y = 6.56 × 10⁻³X − 9.65 × 10⁻⁴	0.9997	1–50	1.0
	Testes	Y = 2.72 × 10⁻³X − 2.72 × 10⁻³	0.9999	1–100	1.0