Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 3

Intraassay and interassay precision and the accuracy of 12 kinds of compounds in rat kidney (n = 6).

Compound	Concentrations (ng/mL)	Intra-day			Inter-day
		Measured (ng/mL)	Precision (%)	Accuracy (RE%)	Measured (ng/mL)	Precision (%)	Accuracy (RE%)
Psoralen	5	4.70 ± 0.26	5.46	−6.04	4.86 ± 0.26	5.45	−2.83
	500	490.30 ± 17.05	3.48	−1.94	492.03 ± 14.95	3.04	−1.59
	800	792.43 ± 11.80	1.49	−0.95	787.82 ± 19.49	2.47	−1.52
Isopsoralen	5	4.75 ± 0.25	5.22	−5.01	4.84 ± 0.25	5.25	−3.14
	500	493.60 ± 20.35	4.12	−1.28	492.37 ± 16.57	3.37	−1.53
	800	782.55 ± 18.84	2.41	−2.18	782.18 ± 14.21	1.82	−2.23
Psoralenoside	12.50	12.37 ± 0.16	1.33	−1.05	12.40 ± 0.13	1.02	−0.78
	1000	956.47 ± 40.02	4.18	−4.35	958.53 ± 37.30	3.89	−4.15
	1600	1565.77 ± 40.30	2.57	−2.14	1551.71 ± 40.97	2.64	−3.02
Bavachin isobavachin	2.50	2.38 ± 0.12	4.86	−4.74	2.41 ± 0.08	3.27	−3.51
	250	243.32 ± 5.69	2.34	−2.67	241.75 ± 4.40	1.82	−3.30
	400	382.78 ± 7.18	1.88	−4.30	388.75 ± 6.77	1.74	−2.81
	0.25	0.24 ± 0.01	2.21	−3.73	0.24 ± 0.01	3.34	−4.56
	125	123.55 ± 1.42	1.15	−1.16	123.13 ± 1.29	1.04	−1.50
	200	190.00 ± 4.23	2.23	−5.00	192.54 ± 4.00	2.08	−3.73
Bavachalcone	1.25	1.23 ± 0.02	1.55	−1.76	1.23 ± 0.02	1.31	−1.72
	50	46.32 ± 1.68	3.63	−7.36	46.79 ± 1.39	2.96	−6.41
	80	74.58 ± 2.14	2.86	−6.78	72.93 ± 3.19	4.38	−8.84
Isobavachalcone	2.50	2.39 ± 0.03	1.30	−4.23	2.38 ± 0.07	2.75	−4.96
	250	247.43 ± 1.24	0.50	−1.03	246.79 ± 3.16	1.28	−1.28
	400	392.60 ± 3.72	0.95	−1.85	394.44 ± 3.11	0.79	−1.39
Pinoresinol diglucoside	2.50	2.29 ± 0.12	5.08	−8.39	2.31 ± 0.18	7.89	−7.79
	50	47.09 ± 1.60	3.41	−5.83	46.35 ± 2.48	5.34	−7.29
	80	75.09 ± 2.48	3.31	−6.14	76.13 ± 2.96	3.88	−4.83
Geniposidic acid	2.50	2.42 ± 0.09	3.87	−3.30	2.36 ± 0.17	7.36	−5.56
	1000	958.65 ± 41.85	4.37	−4.14	966.33 ± 43.26	4.48	−3.37
	1600	1571.00 ± 43.79	2.79	−1.81	1,565.67 ± 43.84	2.80	−2.15
Psoralidin	2.50	2.44 ± 0.09	3.79	−2.55	2.41 ± 0.08	3.45	−3.59
	500	487.13 ± 3.63	0.75	−2.57	483.34 ± 6.97	1.44	−3.33
	800	783.10 ± 9.70	1.24	−2.11	787.11 ± 7.81	0.99	−1.61
Neobavaisoflavone	2.50	2.40 ± 0.03	1.23	−3.97	2.42 ± 0.05	2.04	−3.25
	500	471.82 ± 19.17	4.06	−5.64	478.96 ± 14.06	2.94	−4.21
	800	773.13 ± 14.56	1.88	−3.36	772.81 ± 23.72	3.07	−3.40
Bavachinin	2.50	2.27 ± 0.15	6.68	−9.13	2.33 ± 0.15	6.42	−6.68
	250	246.35 ± 5.10	2.07	−1.46	244.05 ± 6.15	2.52	−2.38
	400	387.50 ± 17.19	4.44	−3.13	385.87 ± 12.18	3.16	−3.53