Journal of Analytical Methods in Chemistry / 2020 / Article / Tab 3 / Research Article
Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats Table 3 Intraassay and interassay precision and the accuracy of 12 kinds of compounds in rat kidney (n = 6).
Compound Concentrations (ng/mL) Intra-day Inter-day Measured (ng/mL) Precision (%) Accuracy (RE%) Measured (ng/mL) Precision (%) Accuracy (RE%) Psoralen 5 4.70 ± 0.26 5.46 −6.04 4.86 ± 0.26 5.45 −2.83 500 490.30 ± 17.05 3.48 −1.94 492.03 ± 14.95 3.04 −1.59 800 792.43 ± 11.80 1.49 −0.95 787.82 ± 19.49 2.47 −1.52 Isopsoralen 5 4.75 ± 0.25 5.22 −5.01 4.84 ± 0.25 5.25 −3.14 500 493.60 ± 20.35 4.12 −1.28 492.37 ± 16.57 3.37 −1.53 800 782.55 ± 18.84 2.41 −2.18 782.18 ± 14.21 1.82 −2.23 Psoralenoside 12.50 12.37 ± 0.16 1.33 −1.05 12.40 ± 0.13 1.02 −0.78 1000 956.47 ± 40.02 4.18 −4.35 958.53 ± 37.30 3.89 −4.15 1600 1565.77 ± 40.30 2.57 −2.14 1551.71 ± 40.97 2.64 −3.02 Bavachin isobavachin 2.50 2.38 ± 0.12 4.86 −4.74 2.41 ± 0.08 3.27 −3.51 250 243.32 ± 5.69 2.34 −2.67 241.75 ± 4.40 1.82 −3.30 400 382.78 ± 7.18 1.88 −4.30 388.75 ± 6.77 1.74 −2.81 0.25 0.24 ± 0.01 2.21 −3.73 0.24 ± 0.01 3.34 −4.56 125 123.55 ± 1.42 1.15 −1.16 123.13 ± 1.29 1.04 −1.50 200 190.00 ± 4.23 2.23 −5.00 192.54 ± 4.00 2.08 −3.73 Bavachalcone 1.25 1.23 ± 0.02 1.55 −1.76 1.23 ± 0.02 1.31 −1.72 50 46.32 ± 1.68 3.63 −7.36 46.79 ± 1.39 2.96 −6.41 80 74.58 ± 2.14 2.86 −6.78 72.93 ± 3.19 4.38 −8.84 Isobavachalcone 2.50 2.39 ± 0.03 1.30 −4.23 2.38 ± 0.07 2.75 −4.96 250 247.43 ± 1.24 0.50 −1.03 246.79 ± 3.16 1.28 −1.28 400 392.60 ± 3.72 0.95 −1.85 394.44 ± 3.11 0.79 −1.39 Pinoresinol diglucoside 2.50 2.29 ± 0.12 5.08 −8.39 2.31 ± 0.18 7.89 −7.79 50 47.09 ± 1.60 3.41 −5.83 46.35 ± 2.48 5.34 −7.29 80 75.09 ± 2.48 3.31 −6.14 76.13 ± 2.96 3.88 −4.83 Geniposidic acid 2.50 2.42 ± 0.09 3.87 −3.30 2.36 ± 0.17 7.36 −5.56 1000 958.65 ± 41.85 4.37 −4.14 966.33 ± 43.26 4.48 −3.37 1600 1571.00 ± 43.79 2.79 −1.81 1,565.67 ± 43.84 2.80 −2.15 Psoralidin 2.50 2.44 ± 0.09 3.79 −2.55 2.41 ± 0.08 3.45 −3.59 500 487.13 ± 3.63 0.75 −2.57 483.34 ± 6.97 1.44 −3.33 800 783.10 ± 9.70 1.24 −2.11 787.11 ± 7.81 0.99 −1.61 Neobavaisoflavone 2.50 2.40 ± 0.03 1.23 −3.97 2.42 ± 0.05 2.04 −3.25 500 471.82 ± 19.17 4.06 −5.64 478.96 ± 14.06 2.94 −4.21 800 773.13 ± 14.56 1.88 −3.36 772.81 ± 23.72 3.07 −3.40 Bavachinin 2.50 2.27 ± 0.15 6.68 −9.13 2.33 ± 0.15 6.42 −6.68 250 246.35 ± 5.10 2.07 −1.46 244.05 ± 6.15 2.52 −2.38 400 387.50 ± 17.19 4.44 −3.13 385.87 ± 12.18 3.16 −3.53