Journal of Analytical Methods in Chemistry / 2020 / Article / Tab 3

Research Article

Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 3

Intraassay and interassay precision and the accuracy of 12 kinds of compounds in rat kidney (n = 6).

CompoundConcentrations (ng/mL)Intra-dayInter-day
Measured (ng/mL)Precision (%)Accuracy (RE%)Measured (ng/mL)Precision (%)Accuracy (RE%)

Psoralen54.70 ± 0.265.46−6.044.86 ± 0.265.45−2.83
500490.30 ± 17.053.48−1.94492.03 ± 14.953.04−1.59
800792.43 ± 11.801.49−0.95787.82 ± 19.492.47−1.52

Isopsoralen54.75 ± 0.255.22−5.014.84 ± 0.255.25−3.14
500493.60 ± 20.354.12−1.28492.37 ± 16.573.37−1.53
800782.55 ± 18.842.41−2.18782.18 ± 14.211.82−2.23

Psoralenoside12.5012.37 ± 0.161.33−1.0512.40 ± 0.131.02−0.78
1000956.47 ± 40.024.18−4.35958.53 ± 37.303.89−4.15
16001565.77 ± 40.302.57−2.141551.71 ± 40.972.64−3.02

Bavachin isobavachin2.502.38 ± 0.124.86−4.742.41 ± 0.083.27−3.51
250243.32 ± 5.692.34−2.67241.75 ± 4.401.82−3.30
400382.78 ± 7.181.88−4.30388.75 ± 6.771.74−2.81
0.250.24 ± 0.012.21−3.730.24 ± 0.013.34−4.56
125123.55 ± 1.421.15−1.16123.13 ± 1.291.04−1.50
200190.00 ± 4.232.23−5.00192.54 ± 4.002.08−3.73

Bavachalcone1.251.23 ± 0.021.55−1.761.23 ± 0.021.31−1.72
5046.32 ± 1.683.63−7.3646.79 ± 1.392.96−6.41
8074.58 ± 2.142.86−6.7872.93 ± 3.194.38−8.84

Isobavachalcone2.502.39 ± 0.031.30−4.232.38 ± 0.072.75−4.96
250247.43 ± 1.240.50−1.03246.79 ± 3.161.28−1.28
400392.60 ± 3.720.95−1.85394.44 ± 3.110.79−1.39

Pinoresinol diglucoside2.502.29 ± 0.125.08−8.392.31 ± 0.187.89−7.79
5047.09 ± 1.603.41−5.8346.35 ± 2.485.34−7.29
8075.09 ± 2.483.31−6.1476.13 ± 2.963.88−4.83

Geniposidic acid2.502.42 ± 0.093.87−3.302.36 ± 0.177.36−5.56
1000958.65 ± 41.854.37−4.14966.33 ± 43.264.48−3.37
16001571.00 ± 43.792.79−1.811,565.67 ± 43.842.80−2.15

Psoralidin2.502.44 ± 0.093.79−2.552.41 ± 0.083.45−3.59
500487.13 ± 3.630.75−2.57483.34 ± 6.971.44−3.33
800783.10 ± 9.701.24−2.11787.11 ± 7.810.99−1.61

Neobavaisoflavone2.502.40 ± 0.031.23−3.972.42 ± 0.052.04−3.25
500471.82 ± 19.174.06−5.64478.96 ± 14.062.94−4.21
800773.13 ± 14.561.88−3.36772.81 ± 23.723.07−3.40

Bavachinin2.502.27 ± 0.156.68−9.132.33 ± 0.156.42−6.68
250246.35 ± 5.102.07−1.46244.05 ± 6.152.52−2.38
400387.50 ± 17.194.44−3.13385.87 ± 12.183.16−3.53

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.