Journal of Analytical Methods in Chemistry / 2020 / Article / Tab 5 / Research Article
Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats Table 5 The stability of 12 compounds in rat kidney (n = 6).
Compound Concentrations (ng/mL) Freeze-thaw stability Short-term stability Long-term stability Preinjection stability Measured (ng/mL) RE (%) Measured (ng/mL) RE (%) Measured (ng/mL) RE (%) Measured (ng/mL) RE (%) Psoralen 5 4.90 ± 0.36 −2.00 4.87 ± 0.12 −2.57 4.96 ± 0.21 −0.90 5.08 ± 0.20 1.60 500 493.95 ± 11.02 −1.21 493.47 ± 13.04 −1.31 501.57 ± 12.64 0.30 497.57 ± 13.3 −0.40 800 752.75 ± 14.76 −5.91 793.47 ± 13.04 −0.82 802.77 ± 13.39 0.35 787.15 ± 23.6 −1.61 Isopsoralen 5 4.88 ± 0.27 −2.50 4.84 ± 0.16 −3.23 4.93 ± 0.21 −1.40 4.98 ± 0.12 −0.37 500 493.12 ± 9.04 −1.38 493.47 ± 13.04 −1.31 498.57 ± 10.47 −0.29 495.07 ± 11.99 −0.99 800 759.58 ± 18.59 −5.05 790.80 ± 13.58 −1.15 801.77 ± 8.19 0.22 792.32 ± 12.03 −0.96 Psoralenoside 12.50 12.30 ± 0.29 −1.60 12.40 ± 0.10 −0.81 12.27 ± 0.10 −1.85 12.37 ± 0.12 −1.03 1000 988.95 ± 11.05 −1.11 990.13 ± 6.60 −0.99 973.80 ± 16.36 −2.62 977.79 ± 12.29 −2.22 1600 1577.75 ± 16.66 −1.39 1586.80 ± 7.10 −0.83 1578.47 ± 7.69 −1.35 1581.0 ± 8.50 −1.19 Bavachin 2.50 2.41 ± 0.06 −3.67 2.36 ± 0.09 −5.45 2.40 ± 0.05 −4.00 2.45 ± 0.05 −2.00 250 243.18 ± 4.68 −2.73 242.37 ± 8.80 −3.05 239.33 ± 7.01 −4.27 247.38 ± 3.65 −1.05 400 384.08 ± 12.05 −3.98 386.93 ± 10.07 −3.27 386.17 ± 5.17 −3.46 391.00 ± 5.33 −2.25 Isobavachin 0.25 0.24 ± 0.01 −3.67 0.24 ± 0.01 −5.37 0.24 ± 0.00 −4.4 0.24 ± 0.01 −4.42 125 123.35 ± 1.13 −1.32 124.37 ± 0.52 −0.51 123.50 ± 0.98 −1.20 123.38 ± 1.89 −1.29 200 197.08 ± 2.84 −1.46 195.08 ± 3.07 −2.46 193.33 ± 2.61 −3.33 196.00 ± 4.29 −2.00 Bavachalcone 1.25 1.24 ± 0.01 −0.73 1.23 ± 0.02 −1.87 1.24 ± 0.01 −1.17 1.23 ± 0.01 −1.27 50 48.40 ± 1.00 −3.2 46.37 ± 1.93 −7.27 45.33 ± 1.97 −9.33 47.32 ± 1.82 −5.37 80 77.08 ± 2.84 −3.65 76.42 ± 2.77 −4.84 75.00 ± 2.63 −6.25 78.50 ± 3.02 −1.88 Isobavachalcone 2.50 2.42 ± 0.03 −3.01 2.45 ± 0.05 −2.09 2.43 ± 0.05 −2.93 2.46 ± 0.05 −1.80 250 242.18 ± 2.93 −3.13 244.85 ± 5.63 −2.06 242.83 ± 3.72 −2.87 247.78 ± 3.67 −0.89 400 388.97 ± 7.41 −2.76 391.27 ± 6.48 −2.18 390.72 ± 6.54 −2.32 391.50 ± 5.85 −2.13 Pinoresinol diglucoside 2.50 2.40 ± 0.07 −4.13 2.43 ± 0.03 −2.67 2.46 ± 0.09 −1.73 2.39 ± 0.12 −4.53 50 49.28 ± 0.59 −1.43 49.30 ± 0.77 −1.40 48.90 ± 1.09 −2.20 49.57 ± 1.35 −0.87 80 77.25 ± 1.68 −3.44 79.23 ± 0.64 −0.97 79.34 ± 0.85 −0.82 78.98 ± 1.60 −1.27 2.5 2.31 ± 0.19 −7.41 2.26 ± 0.11 −0.95 2.28 ± 0.16 −0.87 2.26 ± 0.18 −0.64 1000 969.67 ± 32.73 −3.03 942.67 ± 22.73 −5.73 980.50 ± 24.99 −1.95 923.63 ± 25.24 −7.64 1600 1562.83 ± 39.05 −2.32 1575.83 ± 39.84 −1.51 1570.33 ± 37.45 −1.85 1583.33 ± 24.27 −1.04 Psoralidin 2.50 2.42 ± 0.04 −3.27 2.46 ± 0.04 −1.53 2.45 ± 0.05 −2.20 2.45 ± 0.05 −2.20 500 493.12 ± 8.33 −1.37 494.30 ± 7.37 −1.14 497.23 ± 14.14 −0.55 498.57 ± 12.55 −0.29 800 777.75 ± 9.36 −2.78 797.47 ± 5.18 −0.32 801.10 ± 8.14 0.14 791.62 ± 16.58 −1.05 Psoralen 2.50 2.42 ± 0.06 −3.02 2.27 ± 0.13 −9.19 2.36 ± 0.10 −5.73 2.38 ± 0.11 −4.63 500 467.93 ± 17.07 −6.41 470.27 ± 25.24 −5.95 485.50 ± 19.43 −2.90 466.13 ± 28.44 −6.77 800 760.12 ± 25.96 −4.99 782.12 ± 22.41 −2.24 776.95 ± 17.25 −2.88 768.48 ± 19.69 −3.94 Isopsoralen 2.50 2.40 ± 0.09 −3.87 2.27 ± 0.16 −9.02 2.39 ± 0.13 −4.31 2.42 ± 0.09 −3.26 250 243.68 ± 5.47 −2.53 246.87 ± 18.29 −1.25 235.50 ± 10.69 −5.80 246.55 ± 8.19 −1.38 400 379.92 ± 27.37 −5.02 382.93 ± 24.86 −4.27 384.83 ± 12.14 −3.79 389.33 ± 12.53 −2.67