Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 5

The stability of 12 compounds in rat kidney (n = 6).

Compound	Concentrations (ng/mL)	Freeze-thaw stability		Short-term stability		Long-term stability		Preinjection stability
		Measured (ng/mL)	RE (%)	Measured (ng/mL)	RE (%)	Measured (ng/mL)	RE (%)	Measured (ng/mL)	RE (%)
Psoralen	5	4.90 ± 0.36	−2.00	4.87 ± 0.12	−2.57	4.96 ± 0.21	−0.90	5.08 ± 0.20	1.60
	500	493.95 ± 11.02	−1.21	493.47 ± 13.04	−1.31	501.57 ± 12.64	0.30	497.57 ± 13.3	−0.40
	800	752.75 ± 14.76	−5.91	793.47 ± 13.04	−0.82	802.77 ± 13.39	0.35	787.15 ± 23.6	−1.61
Isopsoralen	5	4.88 ± 0.27	−2.50	4.84 ± 0.16	−3.23	4.93 ± 0.21	−1.40	4.98 ± 0.12	−0.37
	500	493.12 ± 9.04	−1.38	493.47 ± 13.04	−1.31	498.57 ± 10.47	−0.29	495.07 ± 11.99	−0.99
	800	759.58 ± 18.59	−5.05	790.80 ± 13.58	−1.15	801.77 ± 8.19	0.22	792.32 ± 12.03	−0.96
Psoralenoside	12.50	12.30 ± 0.29	−1.60	12.40 ± 0.10	−0.81	12.27 ± 0.10	−1.85	12.37 ± 0.12	−1.03
	1000	988.95 ± 11.05	−1.11	990.13 ± 6.60	−0.99	973.80 ± 16.36	−2.62	977.79 ± 12.29	−2.22
	1600	1577.75 ± 16.66	−1.39	1586.80 ± 7.10	−0.83	1578.47 ± 7.69	−1.35	1581.0 ± 8.50	−1.19
Bavachin	2.50	2.41 ± 0.06	−3.67	2.36 ± 0.09	−5.45	2.40 ± 0.05	−4.00	2.45 ± 0.05	−2.00
	250	243.18 ± 4.68	−2.73	242.37 ± 8.80	−3.05	239.33 ± 7.01	−4.27	247.38 ± 3.65	−1.05
	400	384.08 ± 12.05	−3.98	386.93 ± 10.07	−3.27	386.17 ± 5.17	−3.46	391.00 ± 5.33	−2.25
Isobavachin	0.25	0.24 ± 0.01	−3.67	0.24 ± 0.01	−5.37	0.24 ± 0.00	−4.4	0.24 ± 0.01	−4.42
	125	123.35 ± 1.13	−1.32	124.37 ± 0.52	−0.51	123.50 ± 0.98	−1.20	123.38 ± 1.89	−1.29
	200	197.08 ± 2.84	−1.46	195.08 ± 3.07	−2.46	193.33 ± 2.61	−3.33	196.00 ± 4.29	−2.00
Bavachalcone	1.25	1.24 ± 0.01	−0.73	1.23 ± 0.02	−1.87	1.24 ± 0.01	−1.17	1.23 ± 0.01	−1.27
	50	48.40 ± 1.00	−3.2	46.37 ± 1.93	−7.27	45.33 ± 1.97	−9.33	47.32 ± 1.82	−5.37
	80	77.08 ± 2.84	−3.65	76.42 ± 2.77	−4.84	75.00 ± 2.63	−6.25	78.50 ± 3.02	−1.88
Isobavachalcone	2.50	2.42 ± 0.03	−3.01	2.45 ± 0.05	−2.09	2.43 ± 0.05	−2.93	2.46 ± 0.05	−1.80
	250	242.18 ± 2.93	−3.13	244.85 ± 5.63	−2.06	242.83 ± 3.72	−2.87	247.78 ± 3.67	−0.89
	400	388.97 ± 7.41	−2.76	391.27 ± 6.48	−2.18	390.72 ± 6.54	−2.32	391.50 ± 5.85	−2.13
Pinoresinol diglucoside	2.50	2.40 ± 0.07	−4.13	2.43 ± 0.03	−2.67	2.46 ± 0.09	−1.73	2.39 ± 0.12	−4.53
	50	49.28 ± 0.59	−1.43	49.30 ± 0.77	−1.40	48.90 ± 1.09	−2.20	49.57 ± 1.35	−0.87
	80	77.25 ± 1.68	−3.44	79.23 ± 0.64	−0.97	79.34 ± 0.85	−0.82	78.98 ± 1.60	−1.27
	2.5	2.31 ± 0.19	−7.41	2.26 ± 0.11	−0.95	2.28 ± 0.16	−0.87	2.26 ± 0.18	−0.64
	1000	969.67 ± 32.73	−3.03	942.67 ± 22.73	−5.73	980.50 ± 24.99	−1.95	923.63 ± 25.24	−7.64
	1600	1562.83 ± 39.05	−2.32	1575.83 ± 39.84	−1.51	1570.33 ± 37.45	−1.85	1583.33 ± 24.27	−1.04
Psoralidin	2.50	2.42 ± 0.04	−3.27	2.46 ± 0.04	−1.53	2.45 ± 0.05	−2.20	2.45 ± 0.05	−2.20
	500	493.12 ± 8.33	−1.37	494.30 ± 7.37	−1.14	497.23 ± 14.14	−0.55	498.57 ± 12.55	−0.29
	800	777.75 ± 9.36	−2.78	797.47 ± 5.18	−0.32	801.10 ± 8.14	0.14	791.62 ± 16.58	−1.05
Psoralen	2.50	2.42 ± 0.06	−3.02	2.27 ± 0.13	−9.19	2.36 ± 0.10	−5.73	2.38 ± 0.11	−4.63
	500	467.93 ± 17.07	−6.41	470.27 ± 25.24	−5.95	485.50 ± 19.43	−2.90	466.13 ± 28.44	−6.77
	800	760.12 ± 25.96	−4.99	782.12 ± 22.41	−2.24	776.95 ± 17.25	−2.88	768.48 ± 19.69	−3.94
Isopsoralen	2.50	2.40 ± 0.09	−3.87	2.27 ± 0.16	−9.02	2.39 ± 0.13	−4.31	2.42 ± 0.09	−3.26
	250	243.68 ± 5.47	−2.53	246.87 ± 18.29	−1.25	235.50 ± 10.69	−5.80	246.55 ± 8.19	−1.38
	400	379.92 ± 27.37	−5.02	382.93 ± 24.86	−4.27	384.83 ± 12.14	−3.79	389.33 ± 12.53	−2.67