Journal of Analytical Methods in Chemistry / 2020 / Article / Tab 5

Research Article

Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 5

The stability of 12 compounds in rat kidney (n = 6).

CompoundConcentrations (ng/mL)Freeze-thaw stabilityShort-term stabilityLong-term stabilityPreinjection stability
Measured (ng/mL)RE (%)Measured (ng/mL)RE (%)Measured (ng/mL)RE (%)Measured (ng/mL)RE (%)

Psoralen54.90 ± 0.36−2.004.87 ± 0.12−2.574.96 ± 0.21−0.905.08 ± 0.201.60
500493.95 ± 11.02−1.21493.47 ± 13.04−1.31501.57 ± 12.640.30497.57 ± 13.3−0.40
800752.75 ± 14.76−5.91793.47 ± 13.04−0.82802.77 ± 13.390.35787.15 ± 23.6−1.61

Isopsoralen54.88 ± 0.27−2.504.84 ± 0.16−3.234.93 ± 0.21−1.404.98 ± 0.12−0.37
500493.12 ± 9.04−1.38493.47 ± 13.04−1.31498.57 ± 10.47−0.29495.07 ± 11.99−0.99
800759.58 ± 18.59−5.05790.80 ± 13.58−1.15801.77 ± 8.190.22792.32 ± 12.03−0.96

Psoralenoside12.5012.30 ± 0.29−1.6012.40 ± 0.10−0.8112.27 ± 0.10−1.8512.37 ± 0.12−1.03
1000988.95 ± 11.05−1.11990.13 ± 6.60−0.99973.80 ± 16.36−2.62977.79 ± 12.29−2.22
16001577.75 ± 16.66−1.391586.80 ± 7.10−0.831578.47 ± 7.69−1.351581.0 ± 8.50−1.19

Bavachin2.502.41 ± 0.06−3.672.36 ± 0.09−5.452.40 ± 0.05−4.002.45 ± 0.05−2.00
250243.18 ± 4.68−2.73242.37 ± 8.80−3.05239.33 ± 7.01−4.27247.38 ± 3.65−1.05
400384.08 ± 12.05−3.98386.93 ± 10.07−3.27386.17 ± 5.17−3.46391.00 ± 5.33−2.25

Isobavachin0.250.24 ± 0.01−3.670.24 ± 0.01−5.370.24 ± 0.00−4.40.24 ± 0.01−4.42
125123.35 ± 1.13−1.32124.37 ± 0.52−0.51123.50 ± 0.98−1.20123.38 ± 1.89−1.29
200197.08 ± 2.84−1.46195.08 ± 3.07−2.46193.33 ± 2.61−3.33196.00 ± 4.29−2.00

Bavachalcone1.251.24 ± 0.01−0.731.23 ± 0.02−1.871.24 ± 0.01−1.171.23 ± 0.01−1.27
5048.40 ± 1.00−3.246.37 ± 1.93−7.2745.33 ± 1.97−9.3347.32 ± 1.82−5.37
8077.08 ± 2.84−3.6576.42 ± 2.77−4.8475.00 ± 2.63−6.2578.50 ± 3.02−1.88

Isobavachalcone2.502.42 ± 0.03−3.012.45 ± 0.05−2.092.43 ± 0.05−2.932.46 ± 0.05−1.80
250242.18 ± 2.93−3.13244.85 ± 5.63−2.06242.83 ± 3.72−2.87247.78 ± 3.67−0.89
400388.97 ± 7.41−2.76391.27 ± 6.48−2.18390.72 ± 6.54−2.32391.50 ± 5.85−2.13

Pinoresinol diglucoside2.502.40 ± 0.07−4.132.43 ± 0.03−2.672.46 ± 0.09−1.732.39 ± 0.12−4.53
5049.28 ± 0.59−1.4349.30 ± 0.77−1.4048.90 ± 1.09−2.2049.57 ± 1.35−0.87
8077.25 ± 1.68−3.4479.23 ± 0.64−0.9779.34 ± 0.85−0.8278.98 ± 1.60−1.27
2.52.31 ± 0.19−7.412.26 ± 0.11−0.952.28 ± 0.16−0.872.26 ± 0.18−0.64
1000969.67 ± 32.73−3.03942.67 ± 22.73−5.73980.50 ± 24.99−1.95923.63 ± 25.24−7.64
16001562.83 ± 39.05−2.321575.83 ± 39.84−1.511570.33 ± 37.45−1.851583.33 ± 24.27−1.04

Psoralidin2.502.42 ± 0.04−3.272.46 ± 0.04−1.532.45 ± 0.05−2.202.45 ± 0.05−2.20
500493.12 ± 8.33−1.37494.30 ± 7.37−1.14497.23 ± 14.14−0.55498.57 ± 12.55−0.29
800777.75 ± 9.36−2.78797.47 ± 5.18−0.32801.10 ± 8.140.14791.62 ± 16.58−1.05

Psoralen2.502.42 ± 0.06−3.022.27 ± 0.13−9.192.36 ± 0.10−5.732.38 ± 0.11−4.63
500467.93 ± 17.07−6.41470.27 ± 25.24−5.95485.50 ± 19.43−2.90466.13 ± 28.44−6.77
800760.12 ± 25.96−4.99782.12 ± 22.41−2.24776.95 ± 17.25−2.88768.48 ± 19.69−3.94

Isopsoralen2.502.40 ± 0.09−3.872.27 ± 0.16−9.022.39 ± 0.13−4.312.42 ± 0.09−3.26
250243.68 ± 5.47−2.53246.87 ± 18.29−1.25235.50 ± 10.69−5.80246.55 ± 8.19−1.38
400379.92 ± 27.37−5.02382.93 ± 24.86−4.27384.83 ± 12.14−3.79389.33 ± 12.53−2.67

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