Internal Standards for Quantitative Analysis of Chemical Warfare Agents by the GC/MS Method: Nerve Agents

Table 1

Measurement conditions and parameters.

GC/MS		7890A/5975C	Intuvo 9000/5977B	EM 640
Column		HP-5MS 30 m × 0.25 mm, 0.25 μm	HP-5MS 30 m × 0.25 mm, 0.25 μm	HP-5MS 25 m × 0.35 mm, 1 μm
Carrier gas		Helium, 147 kPa constant pressure	Helium, 1.2 mL/min constant flow	Nitrogen, 500 hPa constant pressure
Sampler		Agilent GC 80	Agilent 7693A	—
Injection volume		1 μl	1 μl	1 μl
Inlet		290°C, splitless mode, purge flow 100 mL/min at 2 min	290°C, splitless mode, purge flow 100 mL/min at 2 min	230°C, splitless mode, purge flow 30 mL/min at 1 min
Oven		40°C (2 min), 10°C/min to 280°C (10 min)	40°C (2 min), 10°C/min to 280°C (10 min)	40°C (2 min), 10°C/min to 280°C (10 min)
Detector		Quadrupole MS, EI, scan mode, transfer line 290°C, scan range 35–600 amu, solvent delay 6 min	Quadrupole MS, EI, scan mode, transfer line 290°C, scan range 35–600 amu, solvent delay 6 min	Quadrupole MS, EI, scan mode, transfer line 280°C, scan range 50–550 amu, solvent delay 6 min
Evaluation of chromatograms		Agilent chemstation GC/MSD—data analysis, version E.02.02., Agilent Technologies, Inc., 2011	MassHunter Workstation software, version B.07.00, Agilent Technologies, Inc., 2014	Bruker Data Analysis, version 1.1., Bruker Daltonik GmbH, 2003