Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves

Figure 3

Chromatogram of extracts of sugarcane, corn, and soybean leaves obtained by sequential injection chromatography. The response for a SA standard is shown for comparison. Sample volume = 10 μL, mobile phase = 7% v/v acetonitrile in 0.2 mol L⁻¹ acetate buffer solution (pH 5.0), flow rate = 10 μL s⁻¹.