Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves

Table 1

Analytical routine for sample preparation in the flow-batch system.

Step	Valve of syringe pump	Selection valve port	Time (s)	Volume (μL)	Flow rate (μL s^−1)
Microwave-assisted extraction
Extractor insertion into reactor	—	—	30	2000	67
Microwave irradiation	—	—	390	—	—
Washing of the extraction channel
Carrier aspiration	In	—	5	1000	200
Extract aspiration	Out	2	12	600	50
Discard to waste	Out	1	8	1600	200
Crude extract preparation
Carrier aspiration	In	—	5	1000	200
Air aspiration	Out	8	10	100	10
Extract aspiration	Out	2	10	100	10
Buffer aspiration	Out	4	5	50	10
HCl aspiration	Out	5	2.5	25	10
NaOH aspiration	Out	3	2.5	25	10
Extract collection	Out	7	37	370	10
Delay	—	—	20	—	—
Channel washing	Out	7	4.65	930	200
Air aspiration	Out	8	10	200	20
Channel empty	Out	7	10	200	20
Hydrolyzed extract preparation
Carrier aspiration	In	—	5	1000	200
Air aspiration	Out	8	10	100	10
NaOH aspiration	Out	3	2.5	25	10
Extract aspiration	Out	2	10	100	10
Transport towards hydrolysis chamber	Out	6	28	280	10
Hydrolysis time	—	—	300	—	—
Hydrolyzed extract aspiration	Out	6	28	280	10
HCl aspiration	Out	5	2.5	25	10
Buffer aspiration	Out	4	5	50	10
Hydrolyzed extract collection	Out	7	37	370	10
Delay	—	—	20	—	—
Channel washing	Out	7	4.65	930	200
Air aspiration	Out	8	10	200	20
Channel empty	Out	7	10	200	20

Extractor insertion with peristaltic pump.