Research Article
Flow-Batch Sample Preparation for Fractionation of the Stress Signaling Phytohormone Salicylic Acid in Fresh Leaves
Table 2
Full-factorial design with coded, real values and analytical responses for robustness evaluation of the procedure for flow-batch alkaline hydrolysis.
| | Experiment | NaOH (mol L−1) | Hydrolysis time (min) | Temperature (°C) | Peak area of total SAa |
| | 1 | −1 (0.09) | −1 (4.5) | −1 (70) | 5.90 | | 2 | 1 (0.11) | −1 (4.5) | −1 (70) | 5.66 | | 3 | −1 (0.09) | 1 (5.5) | −1 (70) | 7.06 | | 4 | 1 (0.11) | 1 (5.5) | −1 (70) | 5.70 | | 5 | −1 (0.09) | −1 (4.5) | 1 (90) | 7.07 | | 6 | 1 (0.11) | −1 (4.5) | 1 (90) | 6.31 | | 7 | −1 (0.09) | 1 (5.5) | 1 (90) | 5.35 | | 8 | 1 (0.11) | 1 (5.5) | 1 (90) | 5.90 |
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aMean values of duplicate measurements.
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